癌症的睡美人小鼠模型：微环境对癌症遗传学的影响。

Guimaraes-Young Amy, Feddersen Charlotte R, Dupuy Adam J

Department of Anatomy and Cell Biology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, United States.

Front Oncol. 2019 Jul 9;9:611. doi: 10.3389/fonc.2019.00611. eCollection 2019.

The Sleeping Beauty (SB) transposon insertional mutagenesis system offers a streamlined approach to identify genetic drivers of cancer. With a relatively random insertion profile, SB is uniquely positioned for conducting unbiased forward genetic screens. Indeed, SB mouse models of cancer have revealed insights into the genetics of tumorigenesis. In this review, we highlight experiments that have exploited the SB system to interrogate the genetics of cancer in distinct biological contexts. We also propose experimental designs that could further our understanding of the relationship between tumor microenvironment and tumor progression.

睡美人（SB）转座子插入诱变系统为鉴定癌症的遗传驱动因素提供了一种简化方法。由于具有相对随机的插入图谱，SB在进行无偏向前向遗传筛选方面具有独特的地位。事实上，SB癌症小鼠模型已经揭示了肿瘤发生遗传学方面的见解。在这篇综述中，我们重点介绍了利用SB系统在不同生物学背景下探究癌症遗传学的实验。我们还提出了一些实验设计，这些设计可以进一步加深我们对肿瘤微环境与肿瘤进展之间关系的理解。