Department of Urology, Hamad Medical Corporation, Doha 00974, Qatar.
Department of Urology, Weill Cornell Medicine-Qatar, Doha 00974, Qatar.
Asian J Androl. 2020 May-Jun;22(3):317-322. doi: 10.4103/aja.aja_75_19.
Oxidative stress (OS) is detrimental to sperm functions, and the oxidation reduction potential (ORP) is a good measure of OS as it considers the balance between oxidants and reductants. Total motile sperm count (TMSC) is viewed as the single most important semen analysis parameter that can predict male infertility severity, and its correlation with ORP has never been undertaken. The objectives of this study were to assess the correlation between ORP and TMSC, to identify the ORP cutoff value based on the TMSC result, and to compare this cutoff value with previously reported ORP cutoff values in literature. One thousand one hundred and sixty-eight infertile patients and 100 fertile controls were enrolled. Demographic and semen data of the participants were retrieved and analyzed. Wilcoxon's rank-sum test compared variables between infertile men and fertile controls; Spearman's correlation assessed the static ORP (sORP)-TMSC relationship for the whole sample and among each group individually. Using a 20×10TMSC threshold, receiver operator characteristic (ROC) analysis determined the sORP cutoff associated with the highest predictive values. TMSC was significantly negatively correlated with sORP across all participants (r = 0.86, P < 0.001), among infertile patients (r = 0.729, P < 0.001), and among fertile controls (r = 0.53, P < 0.001). A 20-million TMSC threshold determined an sORP cutoff value of 2.34 mV/10sperm/ml to be associated with 82.9% sensitivity, 82.8% specificity, 91.5% positive predictive value (PPV), 68.5% negative predictive value (NPV), and 82.9% overall accuracy. Compared with previously reported cutoff values in searched literature, the 2.34 mV/10sperm/ml cutoff value identified in our study yielded the highest overall diagnostic accuracy in the evaluation of infertile men.
氧化应激（OS）对精子功能有害，氧化还原电位（ORP）是衡量 OS 的一个很好的指标，因为它考虑了氧化剂和还原剂之间的平衡。总活动精子计数（TMSC）被视为预测男性不育严重程度的最重要的精液分析参数，而其与 ORP 的相关性从未被研究过。本研究的目的是评估 ORP 与 TMSC 之间的相关性，确定基于 TMSC 结果的 ORP 截断值，并与文献中报道的以前的 ORP 截断值进行比较。共纳入 1168 例不育患者和 100 例正常生育对照者。检索并分析了参与者的人口统计学和精液数据。采用 Wilcoxon 秩和检验比较不育男性和正常生育对照组之间的变量；Spearman 相关分析评估整个样本和各组的静态 ORP（sORP）-TMSC 关系。使用 20×10TMSC 阈值，受试者工作特征（ROC）分析确定与最高预测值相关的 sORP 截断值。所有参与者的 TMSC 与 sORP 呈显著负相关（r = 0.86，P < 0.001），在不育患者中（r = 0.729，P < 0.001）和正常生育对照组中（r = 0.53，P < 0.001）。2000 万 TMSC 阈值确定 sORP 截断值为 2.34 mV/10 个精子/ml，与 82.9%的敏感性、82.8%的特异性、91.5%的阳性预测值（PPV）、68.5%的阴性预测值（NPV）和 82.9%的总准确率相关。与已检索文献中报道的以前的截断值相比，本研究确定的 2.34 mV/10 个精子/ml 截断值在评估不育男性方面具有最高的总体诊断准确性。
