Gill Kamil, Machalowski Tomasz, Harasny Patryk, Kups Michal, Grabowska Marta, Duchnik Ewa, Sipak Olimpia, Fraczek Monika, Kurpisz Maciej, Kurzawa Rafal, Piasecka Malgorzata
Department of Histology and Developmental Biology, Faculty of Health Sciences, Pomeranian Medical University, 71-210 Szczecin, Poland.
Department of Perinatology, Obstetrics and Gynecology, Faculty of Medicine and Dentistry, Pomeranian Medical University, 72-010 Police, Poland.
Antioxidants (Basel). 2022 Oct 5;11(10):1987. doi: 10.3390/antiox11101987.
Our research was designed to verify the relationship between male infertility, basic semen characteristics (with respect to detailed sperm morphology), sperm DNA fragmentation (SDF), oxidation-reduction potential in semen (ORP), and leukocytospermia. The obtained results showed that infertile groups (with or without leukocytospermia) had significantly lower basic semen characteristics and higher SDF, raw ORP, and static ORP (sORP) than fertile controls. The thresholds of 13% SDF (AUC = 0.733) and 1.40 sORP (AUC = 0.857) were predictive values for discriminating infertile from fertile men. In infertile groups, a higher prevalence and risk for >13% SDF and >1.40 sORP were revealed. Unexpectedly, leukocytospermic subjects had lower sORP, prevalence, and risk for >1.40 sORP than leukocytospermic-negative men. These groups did not differ in SDF and raw ORP. Both SDF and sORP negatively correlated with basic semen parameters but positively correlated with sperm head and midpiece defects. sORP positively correlated with sperm tail defects, immature sperm cells with excess residual cytoplasm, and SDF. In turn, raw ORP negatively correlated with sperm count but positively correlated with SDF and sORP. These findings indicate that (1) there is a relationship between male infertility, SDF, and OS in semen; (2) in infertile men, there is a clinically significant risk of SDF and OS irrespective of leukocytospermia; and (3) the assessment of SDF and oxidative stress should be independent of leukocytospermia.
我们的研究旨在验证男性不育、基本精液特征（关于详细的精子形态）、精子DNA碎片化（SDF）、精液中的氧化还原电位（ORP）和白细胞精子症之间的关系。所得结果表明，不育组（有或无白细胞精子症）的基本精液特征显著低于生育对照组，而SDF、原始ORP和静态ORP（sORP）则更高。SDF为13%（AUC = 0.733）和sORP为1.40（AUC = 0.857）的阈值是区分不育男性和生育男性的预测值。在不育组中，SDF>13%和sORP>1.40的患病率和风险更高。出乎意料的是，白细胞精子症患者的sORP、患病率以及sORP>1.40的风险均低于无白细胞精子症的男性。这些组在SDF和原始ORP方面没有差异。SDF和sORP均与基本精液参数呈负相关，但与精子头部和中段缺陷呈正相关。sORP与精子尾部缺陷、有过多残留细胞质的未成熟精子细胞以及SDF呈正相关。反过来，原始ORP与精子计数呈负相关，但与SDF和sORP呈正相关。这些发现表明：（1）男性不育、SDF和精液中的氧化应激之间存在关联；（2）在不育男性中，无论是否存在白细胞精子症，SDF和氧化应激都存在临床上显著的风险；（3）SDF和氧化应激的评估应独立于白细胞精子症。
