Khadhri Ayda, Bouali Intidhar, Aouadhi Chedia, Lagel Marie-Christine, Masson Eric, Pizzi Antonio
Faculty of Sciences, Unit of Research on Vegetal Ecology, Campus Academia, University of El-Manar II, Tunis, Tunisia.
Faculty of Sciences, Unit of Biochemistry of Lipids and Proteins, Campus Academia, University of El-Manar II, Tunis, Tunisia.
Biomed Chromatogr. 2019 Nov;33(11):e4665. doi: 10.1002/bmc.4665. Epub 2019 Sep 1.
This study aimed to investigate the effect of the maturation process of sweet marjoram (Origanum majorana L.) on essential oil composition, the phenolic profile of ethanolic extract and their antioxidant capacities. The essential oil composition was studied at three stages of maturity by GC-MS. Thirty compounds were detected representing 100% of the total essential oil. p-Menth-1-en-4-ol was the major compound (37.15-76.94%) followed by cyclohexanol-3,3,5 trimethyl (5.41-15.99%) and α-terpineol (0.94-11.34%). During the maturation process, an accumulation of oxygenated monoterpenes was observed. The phenolic composition was studied using matrix-assisted laser desorption/ionization time of flight. The analysis showed the presence of short flavonoid monomers at all stages of maturation. The antioxidant capacity of ethanolic extracts and essential oils was evaluated using the DPPH assay, iron chelating power and reducing power assay. The highest phenolic content and antioxidant capacity were found at flowering stage. These findings on essential oil composition, phenolic profile and antioxidant capacity of O. majorana at three different stages of development provide more information on how these secondary metabolites are accumulated.
本研究旨在探究甜牛至（牛至属植物）成熟过程对其精油成分、乙醇提取物的酚类成分及其抗氧化能力的影响。通过气相色谱 - 质谱联用仪（GC - MS）在成熟的三个阶段研究了精油成分。共检测到30种化合物，占精油总量的100%。对 - 薄荷 - 1 - 烯 - 4 - 醇是主要化合物（37.15 - 76.94%），其次是3,3,5 - 三甲基环己醇（5.41 - 15.99%）和α - 松油醇（0.94 - 11.34%）。在成熟过程中，观察到含氧单萜类化合物的积累。使用基质辅助激光解吸/电离飞行时间质谱法研究了酚类成分。分析表明在成熟的各个阶段均存在短黄酮单体。使用DPPH法、铁螯合能力和还原能力测定法评估了乙醇提取物和精油的抗氧化能力。在开花期发现酚类含量和抗氧化能力最高。这些关于牛至在三个不同发育阶段的精油成分、酚类成分和抗氧化能力的研究结果提供了更多关于这些次生代谢产物如何积累的信息。
