猪水疱病病毒三种抗原性颗粒的特性分析

Characterization of three antigenic particles of swine vesicular disease virus.

作者信息

Moore D M

出版信息

J Gen Virol. 1977 Mar;34(3):431-45. doi: 10.1099/0022-1317-34-3-431.

Abstract

Three distinct particles were isolated from cell culture harvests of swine vesicular disease virus (SVDV) by sucrose and CsCl gradient centrifugation. Virions (148S), RNA-free empty capsids (81S), and a third particle (49S) also free of RNA showed immune reactivity with SVDV antiserum. The 81S and 49S particles had polypeptides typical of naturally occurring empty capsids. Injection of purified antigens into guinea pigs produced antisera which distinguished empty capsids from virions on immunodiffusion; the 49S antigen appeared similar to virions. Antisera produced to freshly prepared virus antigen grown in brains of baby mice distinguished SVDV from the serologically related Coxsackie B-5 virus but did not distinguish the individual S particle antigens. Partly purified virus preparations degraded to empty capsids when incubated in guinea pig serum. The possible origin of empty capsids and 49S particles and their relationship to antigenicity of virus preparations are discussed.

摘要

通过蔗糖和氯化铯梯度离心法，从猪水疱病病毒（SVDV）的细胞培养收获物中分离出三种不同的颗粒。病毒粒子（148S）、不含RNA的空衣壳（81S）以及同样不含RNA的第三种颗粒（49S）与SVDV抗血清呈现免疫反应性。81S和49S颗粒具有天然存在的空衣壳典型的多肽。将纯化抗原注射到豚鼠体内产生的抗血清，在免疫扩散试验中能够区分空衣壳和病毒粒子；49S抗原看起来与病毒粒子相似。针对在幼鼠脑中培养的新鲜制备的病毒抗原产生的抗血清，可区分SVDV和血清学相关的柯萨奇B-5病毒，但无法区分各个S颗粒抗原。部分纯化的病毒制剂在豚鼠血清中孵育时会降解为空衣壳。文中讨论了空衣壳和49S颗粒的可能来源及其与病毒制剂抗原性的关系。