巴西首个达林按蚊自由交配实验室种群。
Brazil's first free-mating laboratory colony of Nyssorhynchus darlingi.
作者信息
Araujo Maisa da Silva, Andrade Alice Oliveira, Santos Najara Akira Costa Dos, Pereira Dhélio Batista, Costa Glaucilene da Silva, Paulo Paula Frassinetti Medeiros de, Rios Carlos Tong, Moreno Marta, Pereira-da-Silva Luiz Hidelbrando, Medeiros Jansen Fernandes de
机构信息
Laboratório de Entomologia, Fiocruz Rondônia, Porto Velho, RO, Brasil.
Programa de Pós-Graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia, Porto Velho, RO, Brasil.
出版信息
Rev Soc Bras Med Trop. 2019 Jul 18;52:e20190159. doi: 10.1590/0037-8682-0159-2019.
INTRODUCTION
The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established laboratory colony of Ny. darlingi in Brazil.
METHODS
Mosquitoes were collected from Porto Velho and were reared at the Laboratory of Fiocruz/RO. After induced mating by light stimulation in the F1 to F6, the subsequent generations were free mating. Larvae were reared in distilled water and fed daily until pupation.
RESULTS
In 11 generations, the colony produced a high number of pupae after the F5 generation.
CONCLUSIONS
These results demonstrate the potential for permanently establishing Ny. darlingi colonies for research purposes in Brazil.
引言
高产的达林按蚊实验室种群的缺乏限制了一些研究。我们报告了巴西首个成功建立的达林按蚊实验室种群。
方法
蚊子采自波多韦柳，并在巴西玛瑙斯市奥斯瓦尔多·克鲁兹基金会（Fiocruz/RO）实验室饲养。在F1至F6代通过光刺激诱导交配后，后续世代自由交配。幼虫饲养于蒸馏水中，每天喂食直至化蛹。
结果
在11代中，该种群在F5代之后产生了大量蛹。
结论
这些结果表明在巴西为研究目的永久建立达林按蚊种群具有潜力。
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