Department of Mathematics and Physics "E. De Giorgi", University of Salento, Via Monteroni, 73100 Lecce, Italy.
IIT-Italian Institute of Technology, via Morego 30, 16163 Genova, Italy.
Int J Mol Sci. 2019 Jul 23;20(14):3594. doi: 10.3390/ijms20143594.
The side effects induced by nanoparticle exposure at a cellular level are one of the priority research topics due to the steady increase in the use of nanoparticles (NPs). Recently, the focus on cellular morphology and mechanical behavior is gaining relevance in order to fully understand the cytotoxic mechanisms. In this regard, we have evaluated the morphomechanical alteration in human breast adenocarcinoma cell line (MCF-7) exposed to TiONPs at two different concentrations (25 and 50 µg/mL) and two time points (24 and 48 h). By using confocal and atomic force microscopy, we demonstrated that TiONP exposure induces significant alterations in cellular membrane elasticity, due to actin proteins rearrangement in cytoskeleton, as calculated in correspondence to nuclear and cytoplasmic compartments. In this work, we have emphasized the alteration in mechanical properties of the cellular membrane, induced by nanoparticle exposure.
由于纳米颗粒（NPs）的使用稳步增加，细胞水平上纳米颗粒暴露所引起的副作用是优先研究的课题之一。最近，为了充分了解细胞毒性机制，人们越来越关注细胞形态和机械行为。在这方面，我们评估了暴露于两种不同浓度（25 和 50 µg/mL）和两个时间点（24 和 48 h）的 TiONPs 的人乳腺癌腺癌细胞系（MCF-7）的形态力学变化。通过使用共聚焦和原子力显微镜，我们证明 TiONP 暴露会由于细胞骨架中肌动蛋白蛋白的重排而导致细胞膜弹性发生显著变化，这是根据核和细胞质隔室计算得出的。在这项工作中，我们强调了纳米颗粒暴露引起的细胞膜机械性能的变化。
