Schuetz Mathias, Fidanza Mario, Mattsson Jim
Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada.
Department of Botany, The University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.
Plants (Basel). 2019 Jul 23;8(7):242. doi: 10.3390/plants8070242.
Based on mutant phenotypes the gene acts in several developmental processes including leaf vein development. Since overlapping functions among genes are common, we assessed the related and genes for potential overlap in expression during vein development using in-situ hybridization. Like was expressed in preprocambial and procambial cells. was also expressed in procambial cells, close to and during vein differentiation. was expressed in differentiating vessel elements. To assess if genes with vein expression have overlapping functions, double mutants were generated. While and mutants formed leaves normally, double mutant combinations of with or resulted in a breakdown of leaf formation. Instead, novel structures not present in any of the single mutants formed. The results implicate and in rosette leaf formation and suggest that their functions overlap and act in parallel with in this process. The observed vascular expression patterns suggest unique functions ( and ) and potentially overlapping functions ( and ) in vein development. Since double mutants do not form leaves, assessment of their potential combined action in vein development will require the use of conditional mutants.
基于突变体表型，该基因在包括叶脉发育在内的多个发育过程中发挥作用。由于基因间功能重叠很常见，我们使用原位杂交技术评估了相关基因在叶脉发育过程中表达的潜在重叠情况。与[某基因]一样，[另一基因]在前形成层和形成层细胞中表达。[又一基因]也在形成层细胞中表达，靠近叶脉分化并在叶脉分化期间表达。[再一基因]在分化的导管分子中表达。为了评估具有叶脉表达的基因是否具有重叠功能，我们构建了双突变体。虽然[某基因]和[另一基因]突变体正常形成叶片，但[某基因]与[另一基因]或[又一基因]的双突变体组合导致叶片形成受阻。相反，形成了任何单突变体中都不存在的新结构。结果表明[相关基因]与[另一相关基因]参与莲座叶的形成，并表明它们的功能重叠且在这一过程中与[某基因]平行发挥作用。观察到的维管组织表达模式表明[相关基因]在叶脉发育中具有独特功能（[某基因]和[另一基因]）以及潜在的重叠功能（[某基因]和[另一基因]）。由于[某基因]双突变体不形成叶片，评估它们在叶脉发育中的潜在联合作用将需要使用条件性突变体。
