Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization & National Demonstration Center for Experimental Biology Education & College of Life Sciences, Hubei Normal University, Huangshi 435002, China.
Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China.
Molecules. 2019 Jul 23;24(14):2671. doi: 10.3390/molecules24142671.
As a traditional natural medicine for treating many kinds of diseases, showed apparent inhibition on xanthine oxidase (XO). In this study, ultrafiltration combined with liquid chromatography-mass spectrometry (LC-MS) is used for the screening of XO inhibitors from . Their antioxidation, XO inhibition, and enzymic kinetic parameters are also determined. Finally, piceatannol (), rhaponiticin (), resveratrol (), and isorhapontigenin () are screened out and identified as XO inhibitors from the extract of . Four inhibitors show better inhibition than allopurinol and good radical scavenging abilities. However, the antioxidant activities are weaker than ascorbic acid. The kinetic parameters illustrate the inhibition mode of XO by piceatannol is competitive type, while the inhibition modes for rhaponiticin, resveratrol and isorhapontigenin are uncompetitive types. In order to evaluate the difference among samples obtained in China, the amounts of four inhibitors and related activities in 20 samples are assessed and analyzed by partial least squares analysis. The results indicate piceatannol contribute the highest coefficients in three kinds of activities. Based on these findings, more comprehensive research on pharmaceutical and biochemical activities of these four XO inhibitors could be conducted in future.
作为一种治疗多种疾病的传统天然药物，[草药名称]对黄嘌呤氧化酶（XO）表现出明显的抑制作用。本研究采用超滤结合液质联用（LC-MS）技术筛选[草药名称]中的 XO 抑制剂。并测定其抗氧化、XO 抑制和酶动力学参数。最后，从[草药名称]提取物中筛选并鉴定出白藜芦醇（）、紫檀芪（）、白藜芦醇（）和异落叶松脂素（）为 XO 抑制剂。四种抑制剂对 XO 的抑制作用优于别嘌呤醇，且具有良好的自由基清除能力。然而，其抗氧化活性弱于抗坏血酸。动力学参数表明，白藜芦醇对 XO 的抑制模式为竞争性，而紫檀芪、白藜芦醇和异落叶松脂素的抑制模式为非竞争性。为了评估在中国获得的样品之间的差异，通过偏最小二乘分析对 20 个样品中的四种抑制剂及其相关活性进行了评估和分析。结果表明，在三种活性中，白藜芦醇的系数最高。基于这些发现，未来可以对这四种 XO 抑制剂的药物和生化活性进行更全面的研究。
