Xu W, Zhu H Y, Tong X M, Zhang S Y
Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
Zhonghua Yi Xue Za Zhi. 2020 May 12;100(18):1409-1413. doi: 10.3760/cma.j.cn112137-20191108-02424.
To evaluate the effect of oocyte vitrification on embryo quality and developmental potential. From January 2014 to December 2017, 30 cases of oocytes vitrification in the reproductive center of Sir Run Run Shaw hospital were collected as the frozen group, due to failure of sperm extraction or inability to obtain sufficient sperm at the ovum pick-up-day because of oligoospermia or azoospermia. 220 cases of fresh oocytes from the same period were selected as the fresh group. The fertilization rate, embryo rate, pregnancy rate, implantation rate and live birth rate of the two groups were compared retrospectively. The survival rate of oocyte resuscitation was 91.4% (180/197). In the frozen group, 24 cases were transferred with 14 pregnancies, while in the fresh group, 31 cases were transferred with 18 pregnancies. The number of 2PN fertilized eggs, 2PN embryos and 2PN high-quality embryos in the frozen group was significantly lower than that in the fresh group (3.7±2.5 vs 7.3±4.8), (3.3±2.5 vs 7.2±4.8), and (1.2±1.8 vs 2.9±2.7) (all 0.05). The fertilization rate of the frozen group was 77.2% (115/149) and the high-quality embryo rate was 36.6% (37/101), lower than that of the fresh group 77.6% (1 637/2 109) and 40.9% (651/1 591) (0.05).The pregnancy rate of the frozen group was 58.3% (14/24) higher than that of the fresh group 58.1% (18/31), the implantation rate of each mature oocyte and the live birth rate of each mature oocyte were 10.8% (15/138) and 10.8% (15/138), both higher than that of the fresh group 9.6% (21/218) and 8.7% (19/218) (all 0.05). Vitrification cryopreservation of oocytes may lead to a decrease in embryo quality, but embryo development potential is still considerable. Higher pregnancy rate, implantation rate and live birth rate may be obtained.
评估卵母细胞玻璃化冷冻对胚胎质量及发育潜能的影响。2014年1月至2017年12月，收集浙江大学医学院附属邵逸夫医院生殖中心30例因取精失败或因少弱精、无精症在取卵日未能获取足够精子而行卵母细胞玻璃化冷冻的患者作为冷冻组。同期选取220例行新鲜卵母细胞的患者作为新鲜组。回顾性比较两组的受精率、胚胎率、妊娠率、种植率及活产率。卵母细胞复苏存活率为91.4%（180/197）。冷冻组移植24例，妊娠14例；新鲜组移植31例，妊娠18例。冷冻组2PN受精卵、2PN胚胎及2PN优质胚胎数量均显著低于新鲜组（3.7±2.5 vs 7.3±4.8）、（3.3±2.5 vs 7.2±4.8）及（1.2±1.8 vs 2.9±2.7）（均P<0.05）。冷冻组受精率为77.2%（115/149），优质胚胎率为36.6%（37/101），低于新鲜组的77.6%（1637/2109）及40.9%（651/1591）（P<0.05）。冷冻组妊娠率为58.3%（14/24），高于新鲜组的58.1%（18/31）；冷冻组每个成熟卵母细胞的种植率及活产率分别为10.8%（15/138）及10.8%（15/138），均高于新鲜组的9.6%（21/218）及8.7%（19/218）（均P<0.05）。卵母细胞玻璃化冷冻可能导致胚胎质量下降，但胚胎发育潜能仍可观，可获得较高的妊娠率、种植率及活产率。
