建立一种基于单克隆抗体的酶联免疫吸附分析方法用于小麦籽粒中多效唑残留量的检测。
Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of paclobutrazol residue in wheat kernel.
机构信息
Engineering Research Center of Plant Growth Regulator, Ministry of Education, College of Agronomy and Biotechnology and §College of Science, China Agricultural University , Beijing 100193, China.
出版信息
J Agric Food Chem. 2014 Feb 26;62(8):1826-31. doi: 10.1021/jf404905w. Epub 2014 Feb 18.
An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with monoclonal antibody (mAb) mAb6H73C9 recognizing the plant growth regulator paclobutrazol (PBZ). The icELISA had a half-maximum inhibition concentration (IC50) and working range of approximately 8.7 and 2.0-50.4 ng/mL, respectively. Average recoveries of PBZ in the wheat (Triticum aestivum) kernel samples were between 84.3 and 118.9% with relative standard deviations between 3.9 and 14.2%. As determined by the icELISA and further confirmed by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS) analysis, the maximum residue concentration was about 0.07 mg/kg in the kernel samples, which indicated that PBZ could transfer from PBZ- treated seedlings to the kernel samples. The correlation coefficient (R(2)) between icELISA and LC-ESI-MS results was 0.979, which manifested that the developed icELISA was sensitive enough for monitoring PBZ residues in wheat kernels.
建立了一种用于检测植物生长调节剂多效唑（PBZ）的间接竞争酶联免疫吸附分析方法（icELISA），该方法使用单克隆抗体 mAb6H73C9 作为检测抗体，其识别 PBZ。icELISA 的半抑制浓度（IC50）和工作范围分别约为 8.7 和 2.0-50.4ng/mL。在小麦（Triticum aestivum）籽粒样品中，PBZ 的平均回收率在 84.3%至 118.9%之间，相对标准偏差在 3.9%至 14.2%之间。通过 icELISA 测定，并通过液相色谱-电喷雾电离四极杆轨道阱质谱（LC-ESI-MS）分析进一步证实，籽粒样品中的最大残留浓度约为 0.07mg/kg，表明 PBZ 可以从 PBZ 处理的幼苗转移到籽粒样品中。icELISA 和 LC-ESI-MS 结果之间的相关系数（R2）为 0.979，表明所建立的 icELISA 足以用于监测小麦籽粒中的 PBZ 残留。
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