Engineering Research Center of Plant Growth Regulator, Ministry of Education, College of Agronomy and Biotechnology and §College of Science, China Agricultural University , Beijing 100193, China.
J Agric Food Chem. 2014 Feb 26;62(8):1826-31. doi: 10.1021/jf404905w. Epub 2014 Feb 18.
An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with monoclonal antibody (mAb) mAb6H73C9 recognizing the plant growth regulator paclobutrazol (PBZ). The icELISA had a half-maximum inhibition concentration (IC50) and working range of approximately 8.7 and 2.0-50.4 ng/mL, respectively. Average recoveries of PBZ in the wheat (Triticum aestivum) kernel samples were between 84.3 and 118.9% with relative standard deviations between 3.9 and 14.2%. As determined by the icELISA and further confirmed by liquid chromatography-electrospray ionization quadrupole Orbitrap mass spectrometry (LC-ESI-MS) analysis, the maximum residue concentration was about 0.07 mg/kg in the kernel samples, which indicated that PBZ could transfer from PBZ- treated seedlings to the kernel samples. The correlation coefficient (R(2)) between icELISA and LC-ESI-MS results was 0.979, which manifested that the developed icELISA was sensitive enough for monitoring PBZ residues in wheat kernels.
建立了一种用于检测植物生长调节剂多效唑（PBZ）的间接竞争酶联免疫吸附分析方法（icELISA），该方法使用单克隆抗体 mAb6H73C9 作为检测抗体，其识别 PBZ。icELISA 的半抑制浓度（IC50）和工作范围分别约为 8.7 和 2.0-50.4ng/mL。在小麦（Triticum aestivum）籽粒样品中，PBZ 的平均回收率在 84.3%至 118.9%之间，相对标准偏差在 3.9%至 14.2%之间。通过 icELISA 测定，并通过液相色谱-电喷雾电离四极杆轨道阱质谱（LC-ESI-MS）分析进一步证实，籽粒样品中的最大残留浓度约为 0.07mg/kg，表明 PBZ 可以从 PBZ 处理的幼苗转移到籽粒样品中。icELISA 和 LC-ESI-MS 结果之间的相关系数（R2）为 0.979，表明所建立的 icELISA 足以用于监测小麦籽粒中的 PBZ 残留。
