Lovett J
Food and Drug Administration, Division of Microbiology, Cincinnati, OH 45226.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):658-60.
After an outbreak of listeriosis in Massachusetts in 1983, the ability of Listeria monocytogenes to survive in raw and pasteurized milk was investigated. An enrichment broth (EB) containing acriflavine, nalidixic acid, and cycloheximide was used to eliminate overgrowth of the culture by competing organisms, and a modification of McBride's agar (MMA) was used as the isolation medium. The culture was incubated 24 h at 30 degrees C. To isolate Listeria from soft cheese, the incubation period was lengthened to 1 week, and the EB culture was streaked to MMA at 1 and 7 days. Physical and biochemical patterns, the CAMP test, serological tests, and mouse pathogenicity studies were helpful in determining the identity of L. monocytogenes.
1983年马萨诸塞州爆发李斯特菌病后，对单核细胞增生李斯特菌在生牛奶和巴氏杀菌牛奶中的存活能力进行了调查。使用含有吖啶黄素、萘啶酸和放线菌酮的富集肉汤（EB）来消除竞争微生物对培养物的过度生长，并使用改良的麦克布赖德琼脂（MMA）作为分离培养基。将培养物在30℃下孵育24小时。为了从软奶酪中分离李斯特菌，孵育期延长至1周，并在第1天和第7天将EB培养物划线接种到MMA上。物理和生化特征、CAMP试验、血清学试验以及小鼠致病性研究有助于确定单核细胞增生李斯特菌的身份。
