Princess Margaret Cancer Centre, University Health Network, 101 College Street, PMCRT 5-354, Toronto, ON, M5G 1L7, Canada.
Institute of Biomaterials and Biomedical Engineering, University of Toronto, 64 College St., Toronto, ON, M5S 3G9, Canada.
Angew Chem Int Ed Engl. 2019 Sep 16;58(38):13394-13399. doi: 10.1002/anie.201907754. Epub 2019 Aug 13.
Organic building blocks are the centerpieces of "one-for-all" nanoparticle development. Herein, we report the synthesis of a novel aza-BODIPY-lipid building block and its self-assembly into a liposomal nanoparticle (BODIPYsome). We observed optically stable NIR J-aggregation within the BODIPYsome that is likely attributed to J-dimerization. BODIPYsomes with cholesterol showed enhanced colloidal stability while maintaining a high extinction coefficient (128 mm cm ) and high fluorescence quenching (99.70±0.09 %), which enables photoacoustic (PA) properties from its intact structure and recovered NIR fluorescence properties when it is disrupted in cancer cells. Finally, its capabilities for optical imaging (PA/fluorescence) were observed in an orthotopic prostate tumor mouse model 24 h after intravenous administration. Overall, the BODIPYsome opens the door for engineering new building blocks in the design of optically stable biophotonic imaging agents.
有机砌块是“一专多能”纳米粒子发展的核心。在此，我们报告了一种新型氮杂 BODIPY-脂质砌块的合成及其自组装成脂质体纳米颗粒（BODIPYsome）。我们观察到 BODIPYsome 内具有光学稳定性的近红外 J-聚集，这可能归因于 J-二聚化。具有胆固醇的 BODIPYsomes 保持了高消光系数（128mm cm ）和高荧光猝灭（99.70±0.09 %），同时具有增强的胶体稳定性，这使其在完整结构时具有光声（PA）特性，而当其在癌细胞中被破坏时又能恢复近红外荧光特性。最后，在静脉注射后 24 小时，在原位前列腺肿瘤小鼠模型中观察到其光学成像（PA/荧光）的能力。总的来说，BODIPYsome 为设计具有光学稳定性的生物光子成像剂的新型砌块开辟了道路。
