Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Methods Enzymol. 2021;657:415-441. doi: 10.1016/bs.mie.2021.06.022. Epub 2021 Jul 21.
In this chapter, we describe a generalizable strategy to obtain a high PA output platform that is optimized for ratiometric imaging. Our approach entails conformationally restricting pendant aryl rings on the aza-BODIPY core to enhance orbital overlap which consequently increases the extinction coefficient. This strategy can potentially be applied to other dye platforms to enhance their signal intensity. We provide detailed protocols for the synthesis, in vitro characterization, and in vivo application.
在本章中，我们描述了一种可推广的策略，以获得针对比率成像进行了优化的高 PA 输出平台。我们的方法涉及对氮杂-BODIPY 核心上的芳基侧链进行构象限制，以增强轨道重叠，从而增加消光系数。该策略可潜在应用于其他染料平台以增强其信号强度。我们提供了详细的合成、体外表征和体内应用的方案。
