Department of Thyroid Surgery, The First Hospital of Jilin University, 71 Xinmin Street, Changchun, Jilin 130021, PR China; State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, PR China.
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
J Colloid Interface Sci. 2019 Feb 15;536:208-214. doi: 10.1016/j.jcis.2018.10.051. Epub 2018 Oct 19.
Theranostics, integrating tumor treatment and diagnosis concurrently, has become an emerging and meaningful strategy in cancer therapy. In this work, an amphiphilic redox-sensitive near-infrared (NIR) BODIPY dye, which could be formed into nanoparticles (PEG-SS-BDP NPs) by self-assembly, was synthesized and possessed good capability of photothermal therapy (PTT), near-infrared fluorescence (NIRF) imaging, photoacoustic (PA) imaging and drug loading. The stable nanoparticles could be dissociated to turn on NIRF due to the rift of embedded disulfide bonds by glutathione (GSH). The enhanced fluorescence in vitro could be observed via confocal laser scanning microscopy (CLSM) after adding GSH, confirming the redox-sensitivity of disulfide bonds. NIRF and PA imaging demonstrated active accumulation in tumor and good imaging effect. At last, PEG-SS-BDP NPs could significantly suppress tumor growth in vivo upon irradiation. The amphiphilic redox-sensitive BODIPY nanoparticles provide a promising design strategy to formulate multifunctional stimuli-responsive theranostic nanoplatforms.
治疗学，即将肿瘤治疗与诊断相结合，已成为癌症治疗中一种新兴且有意义的策略。在这项工作中，我们合成了一种具有两亲性的氧化还原敏感近红外（NIR）BODIPY 染料，它可以通过自组装形成纳米颗粒（PEG-SS-BDP NPs），具有良好的光热治疗（PTT）、近红外荧光（NIRF）成像、光声（PA）成像和药物负载能力。由于嵌入的二硫键断裂，稳定的纳米颗粒在谷胱甘肽（GSH）的作用下可以解离，从而开启 NIRF。通过共聚焦激光扫描显微镜（CLSM）观察到加入 GSH 后体外荧光增强，证实了二硫键的氧化还原敏感性。NIRF 和 PA 成像显示肿瘤的主动积累和良好的成像效果。最后，在照射下，PEG-SS-BDP NPs 可以显著抑制体内肿瘤生长。具有两亲性氧化还原敏感的 BODIPY 纳米颗粒为构建多功能刺激响应性治疗学纳米平台提供了一种有前途的设计策略。
