Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China; College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.
Colloids Surf B Biointerfaces. 2019 Oct 1;182:110367. doi: 10.1016/j.colsurfb.2019.110367. Epub 2019 Jul 15.
Effective hemostasis improvements for penetrating traumas remain a research priority for civilian and noncivilian applications. Herein, we fabricated an expandable keratin sponge (EKS) for the hemostatic treatment of a penetrating trauma based on the excellent hemostatic ability of keratin and the expandable property of polyacrylamide (PAM). EKSs with semi-interpenetrating networks were fabricated by radical polymerization of keratin and PAM, and the EKS showed rapid expansion upon blood absorption. This sponge exhibited effective hemostasis on a rat penetrating liver hemorrhage, and the expansion of the EKS was dependent on the bleeding volume. In addition, the results of a shear wave elastography analysis showed that the elasticity of the liver tissue increased from 12.5 kPa to 21.2 kPa after the penetrating liver trauma treated by the EKS, and the mechanical strength of the liver tissue was maintained after 1 h of the EKS application. Further in vivo tests indicated the effectiveness of the EKS for hemostasis in a swine femoral artery transection hemorrhage model. This EKS is promising for hemostatic applications.
对于民用和非民用应用，提高穿透性创伤的有效止血仍然是研究重点。在此，我们基于角蛋白的优异止血能力和聚丙烯酰胺（PAM）的可膨胀特性，为穿透性创伤的止血治疗制造了一种可膨胀角蛋白海绵（EKS）。通过角蛋白和 PAM 的自由基聚合制造了具有半互穿网络的 EKS，并且 EKS 在吸收血液时会迅速膨胀。该海绵在大鼠穿透性肝出血模型中表现出有效的止血作用，并且 EKS 的膨胀取决于出血量。此外，剪切波弹性成像分析的结果表明，用 EKS 处理穿透性肝损伤后，肝组织的弹性从 12.5kPa 增加到 21.2kPa，并且在 EKS 应用 1 小时后肝组织的机械强度得以维持。进一步的体内试验表明，EKS 在猪股动脉横断出血模型中止血是有效的。这种 EKS 在止血应用方面具有广阔的前景。
