Marine Pollution Department, Environmental Division, National Institute of Oceanography and Fisheries, Kayet Bey, Elanfoushy, Alexandria, Egypt.
Fabrication Technology Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria, Egypt.
Sci Rep. 2022 Sep 14;12(1):15460. doi: 10.1038/s41598-022-19390-3.
Electrospinning nanofibers have a tremendous interest in biomedical applications such as tissue engineering, drug administration, and wound healing because of their ability to replicate and restore the function of the natural extracellular matrix found in tissues. The study's highlight is the electrospinning preparation and characterization of polyacrylonitrile with chicken feather keratin as an additive. In this study, keratin was extracted from chicken feather waste using an environmentally friendly method and used to reinforce polymeric nanofiber mats. Scanning electron microscopy, energy dispersive spectroscopy, and transmission electron microscopy were used to examine the morphology and the structure of the prepared nanofiber mats. The effect of keratin on the porosity and the tensile strength of reinforcing nanofibers is investigated. The porosity ratio of the nanofiber mats goes up from 24.52 ± 2.12 for blank polyacrylonitrile (PAN (NF)) to 90.89 ± 1.91% for polyacrylonitrile nanofiber with 0.05 wt% keratin (PAN/0.05% K). Furthermore, keratin reinforcement improves the nanofiber's mechanical properties, which are important for wound dressing application, as well as its antibacterial activity without causing hemolysis (less than 2%). The best antibacterial activities were observed against Pseudomonas aeruginosa (30 ± 0.17 mm inhibition zone) and Staphylococcus aureus (29 ± 0.31 mm inhibition zone) for PAN/0.05% K sample, according to the antibacterial test. This research has a good potential to broaden the use of feather keratin-based nanofibers in wound healing.
静电纺丝纳米纤维在生物医学应用中具有巨大的兴趣，如组织工程、药物管理和伤口愈合，因为它们能够复制和恢复组织中天然细胞外基质的功能。本研究的重点是使用一种环保的方法从鸡毛废料中提取角蛋白，并将其作为添加剂来增强聚合物纳米纤维垫。扫描电子显微镜、能谱和透射电子显微镜用于研究制备的纳米纤维垫的形态和结构。研究了角蛋白对增强纳米纤维的孔隙率和拉伸强度的影响。纳米纤维垫的孔隙率比从空白聚丙烯腈（PAN（NF））的 24.52±2.12%增加到 0.05 wt%角蛋白增强的聚丙烯腈纳米纤维（PAN/0.05%K）的 90.89±1.91%。此外，角蛋白增强提高了纳米纤维的机械性能，这对于伤口敷料应用很重要，同时还具有抗菌活性，不会引起溶血（小于 2%）。根据抗菌试验，PAN/0.05%K 样品对铜绿假单胞菌（30±0.17mm 抑菌圈）和金黄色葡萄球菌（29±0.31mm 抑菌圈）具有最好的抗菌活性。这项研究为基于羽毛角蛋白的纳米纤维在伤口愈合中的应用提供了广阔的前景。
