School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, 270 Xueyuan West Road, Wenzhou, Zhejiang 325027, China.
Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, 1 Yonglian Street, Wenzhou, Zhejiang 325000, China.
Biomacromolecules. 2024 Jul 8;25(7):3930-3945. doi: 10.1021/acs.biomac.4c00084. Epub 2024 May 31.
The development of a natural, additive-free, absorbable sponge with procoagulant activity for noncompressible hemostasis remains a challenging task. In this study, we extracted high molecular weight keratin (HK) from human hair and transformed it into a hemostatic sponge with a well-interconnected pore structure using a foaming technique, freeze-drying, and oxidation cross-linking. By controlling the cross-linking degree, the resulting sponge demonstrated excellent liquid absorption ability, shape recovery characteristics, and robust mechanical properties. The HK10 sponge exhibited rapid liquid absorption, expanding up to 600% within 5 s. Moreover, the HK sponge showed superior platelet activation and blood cell adhesion capabilities. In SD rat liver defect models, the sponges demonstrated excellent hemostatic performance by sealing the wound and expediting coagulation, reducing the hemostatic time from 825 to 297 s. Furthermore, HK sponges have excellent biosafety, positioning them as a promising absorbable sponge with the potential for the treatment of noncompressible hemostasis.
从人发中提取高分子量角蛋白（HK），通过发泡技术、冷冻干燥和氧化交联将其转化为具有良好互联孔结构的止血海绵。通过控制交联度，得到的海绵表现出优异的液体吸收能力、形状恢复特性和强大的机械性能。HK10 海绵表现出快速的液体吸收能力，在 5 秒内膨胀高达 600%。此外，HK 海绵显示出优越的血小板激活和血细胞黏附能力。在 SD 大鼠肝缺损模型中，该海绵通过封闭伤口和加速凝血，表现出优异的止血性能，将止血时间从 825 秒缩短至 297 秒。此外，HK 海绵具有良好的生物安全性，有望成为一种可吸收的止血海绵，用于治疗非压迫性止血。
