Ax R L, Dickson K, Lenz R W
J Dairy Sci. 1985 Feb;68(2):387-90. doi: 10.3168/jds.S0022-0302(85)80836-5.
This study was designed to relate nonreturn rates to induction of acrosome reactions by the glycosaminoglycan chondroitin sulfate. Semen samples were obtained from four bulls above and four bulls below 65% nonreturn. Washed sperm from four ejaculates from each bull were incubated for 9 h with 0, 1, 10, or 50 micrograms/ml chondroitin sulfate. Acrosome reactions were evaluated by microscopy with Nomarski optics. Sperm from the higher fertility bulls exhibited significantly higher percentages of acrosome reactions to 10 and 50 micrograms/ml of chondroitin sulfate, and the slope of their dose-response regression line was significantly greater.
本研究旨在探讨硫酸软骨素这种糖胺聚糖诱导顶体反应与不返情率之间的关系。从不返情率高于65%的4头公牛和不返情率低于65%的4头公牛采集精液样本。将每头公牛的4次射精所获得的洗涤精子与0、1、10或50微克/毫升的硫酸软骨素一起孵育9小时。使用微分干涉差显微镜通过显微镜检查评估顶体反应。来自繁殖力较高公牛的精子对10和50微克/毫升硫酸软骨素表现出明显更高比例的顶体反应，并且其剂量反应回归线的斜率明显更大。
