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缅甸禽类中鸡毒支原体、滑液囊支原体和传染性支气管炎病毒的分子检测与遗传特征分析。

Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar.

机构信息

Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

University of Veterinary Science, Yezin, Nay Pyi Taw, Myanmar.

出版信息

BMC Vet Res. 2019 Jul 25;15(1):261. doi: 10.1186/s12917-019-2018-2.

DOI:10.1186/s12917-019-2018-2
PMID:31345206
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6659308/
Abstract

BACKGROUND

In Southeast Asian countries, including Myanmar, poultry farming is a major industry. In order to manage and maintain stable productivity, it is important to establish policies for biosecurity. Infectious respiratory diseases are a major threat to poultry farming. Avian influenza and Newcastle disease have been reported in Myanmar, but no scientific information is available for other respiratory pathogens, such as mycoplasmas and infectious bronchitis virus (IBV). Identifying the genotypes and serotypes of IBVs is especially important to inform vaccination programs. In this study, we detected Mycoplasma gallisepticum (MG), M. synoviae (MS), and IBV in several poultry farms in Myanmar.

RESULTS

Samples were collected from 20 farms in three major poultry farming areas in Myanmar, and MG, MS, and IBV were detected on two, four, and eight farms, respectively, by polymerase chain reaction. Phylogenetic analysis revealed that the observed MG and MS isolates were not identical to vaccine strains. Three different genotypes of IBV were detected, but none was an unknown variant.

CONCLUSIONS

Mycoplasmas and IBV were detected on poultry farms in Myanmar. Periodic surveillance is required to establish the distribution of each pathogen, and to institute better vaccine protocols.

摘要

背景

在包括缅甸在内的东南亚国家,家禽养殖业是一个主要产业。为了管理和维持稳定的生产力,制定生物安全政策很重要。传染性呼吸道疾病是家禽养殖业的主要威胁。据报道,缅甸发生了禽流感和新城疫,但其他呼吸道病原体,如支原体和传染性支气管炎病毒(IBV),尚无科学信息。确定 IBV 的基因型和血清型对于疫苗接种计划尤为重要。在这项研究中,我们在缅甸的几个家禽养殖场中检测到了鸡毒支原体(MG)、滑液支原体(MS)和 IBV。

结果

从缅甸三个主要家禽养殖区的 20 个农场采集样本,通过聚合酶链反应分别在两个、四个和八个农场检测到 MG、MS 和 IBV。系统进化分析表明,观察到的 MG 和 MS 分离株与疫苗株不同。检测到三种不同基因型的 IBV,但均不是未知变体。

结论

在缅甸的家禽养殖场中检测到了支原体和 IBV。需要定期进行监测,以确定每种病原体的分布情况,并制定更好的疫苗方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e64/6659308/295a997b95c7/12917_2019_2018_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e64/6659308/a46075e1040e/12917_2019_2018_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e64/6659308/321f682d182d/12917_2019_2018_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e64/6659308/295a997b95c7/12917_2019_2018_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e64/6659308/a46075e1040e/12917_2019_2018_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e64/6659308/321f682d182d/12917_2019_2018_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e64/6659308/295a997b95c7/12917_2019_2018_Fig3_HTML.jpg

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