濒危瑞香科植物及相关物种微卫星标记的特征分析
Characterization of microsatellite markers for the endangered (Thymelaeaceae) and related species.
作者信息
García-Verdugo Carlos, Illera Juan Carlos, Traveset Anna
机构信息
Dept. de Biología Universitat de les Illes Balears Ctra. Valldemossa Km 7.5 Palma 07122 Balearic Islands Spain.
Jardín Botánico Atlántico - Universidad de Oviedo Avda. del Jardín Botánico 2230 33203 Gijón/Xixón Spain.
出版信息
Appl Plant Sci. 2019 Jul 11;7(7):e11274. doi: 10.1002/aps3.11274. eCollection 2019 Jul.
PREMISE
The endangered shrub (Thymelaeaceae) is endemic to the Balearic island of Menorca, where fragmentation and severe population decline are ongoing threats to this taxon. We developed a set of microsatellite markers to analyze the fine-scale genetics of its few extant populations.
METHODS AND RESULTS
Fifteen microsatellite markers were obtained through Illumina high-throughput sequencing and tested in two populations. Twelve of these loci showed no evidence of null alleles and were highly polymorphic, with a mean number of 8.3 alleles per locus. Levels of observed and expected heterozygosity ranged from 0.100 to 0.952 and from 0.095 to 0.854, respectively. Seven to nine of these loci were successfully amplified in five other species.
CONCLUSIONS
This set of markers provides a useful tool for investigating the factors driving fine-scale population structure in this threatened species, and it represents a novel genetic resource for other European species.
前提
这种濒危灌木（瑞香科）是梅诺卡岛巴利阿里群岛特有的，在该岛，种群碎片化和严重衰退对这个分类群构成持续威胁。我们开发了一组微卫星标记，以分析其现存少数种群的精细尺度遗传学。
方法与结果
通过Illumina高通量测序获得了15个微卫星标记，并在两个种群中进行了测试。其中12个位点没有无效等位基因的证据，且具有高度多态性，每个位点平均有8.3个等位基因。观察到的杂合度和预期杂合度水平分别为0.100至0.952和0.095至0.854。这些位点中的7至9个在其他5个物种中成功扩增。
结论
这组标记为研究驱动这种濒危物种精细尺度种群结构的因素提供了有用工具，并且它代表了其他欧洲物种的一种新的遗传资源。
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