为古老特有针叶树（罗汉松科）开发15个核EST微卫星标记。
Development of 15 nuclear EST microsatellite markers for the paleoendemic conifer (Podocarpaceae).
作者信息
Worth James R P, Marthick James R, Rossetto Maurizio, Cohen Joel, Bourke Greg, Jordan Gregory J
机构信息
Department of Forest Molecular Genetics and Biotechnology Forestry and Forest Products Research Institute Matsunosato 1 Tsukuba Ibaraki 305-8687 Japan.
Menzies Institute for Medical Research University of Tasmania Hobart Tasmania Australia.
出版信息
Appl Plant Sci. 2018 Jun 26;6(6):e01160. doi: 10.1002/aps3.1160. eCollection 2018 Jun.
PREMISE OF THE STUDY
Nuclear microsatellite markers were developed for population genetic analysis of the threatened paleoendemic conifer (Podocarpaceae).
METHODS AND RESULTS
Fifteen variable loci were identified showing one to 13 alleles per population, with seven loci displaying at least four alleles in all populations, and the average number of alleles per locus ranging from 4.80 to 5.93 per population. Levels of observed heterozygosity per locus varied from 0.00 to 0.91, while average heterozygosity across all loci varied from 0.54 to 0.63 between populations. All loci also amplified in the endangered congener , but only five of the loci had more than one allele.
CONCLUSIONS
These 15 loci are the first microsatellite markers developed in the genus . These loci will be useful for investigating the species' extant genetic diversity and structure, the impact of past environmental change, and the significance of asexual reproduction.
研究前提
开发了核微卫星标记，用于对濒危古特有针叶树（罗汉松科）进行群体遗传分析。
方法与结果
鉴定出15个可变位点，每个群体显示1至13个等位基因，7个位点在所有群体中显示至少4个等位基因，每个群体中每个位点的等位基因平均数为4.80至5.93。每个位点的观察杂合度水平在0.00至0.91之间变化，而所有位点的平均杂合度在群体间从0.54至0.63不等。所有位点在濒危同属物种中也能扩增，但只有5个位点有不止一个等位基因。
结论
这15个位点是该属中开发的首批微卫星标记。这些位点将有助于研究该物种现存的遗传多样性和结构、过去环境变化的影响以及无性繁殖的意义。
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