Colgrave Michelle L, Allingham Peter G, Tyrrell Kerri, Jones Alun
Agriculture and Food, CSIRO, St Lucia, QLD, Australia.
Cooperative Research Centre for Sheep Industry Innovation, CJ Hawkins Homestead, University of New England, Armidale, NSW, Australia.
Methods Mol Biol. 2019;2030:33-45. doi: 10.1007/978-1-4939-9639-1_4.
Multiple reaction monitoring (MRM) mass spectrometry may be regarded as the gold standard methodology for quantitative mass spectrometry and has been adopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to measurement of the basic building blocks of proteins, amino acids. Here we describe the application of MRM mass spectrometry to the measurement of hydroxyproline after acid hydrolysis of various animal tissues. We show that measurement of hydroxyproline provides an accurate and reliable estimate of the collagen content of such tissues and may be a useful indicator of meat tenderness.
多反应监测（MRM）质谱分析法可被视为定量质谱分析的金标准方法，已被应用于小分子分析，尤其是在制药行业。它也可用于肽和蛋白质的分析以及蛋白质基本组成部分氨基酸的测量。在此，我们描述了多反应监测质谱分析法在各种动物组织酸水解后羟脯氨酸测量中的应用。我们表明，羟脯氨酸的测量为这些组织的胶原蛋白含量提供了准确可靠的估计，并且可能是肉嫩度的一个有用指标。
