Lab. de Physique des Interfaces et des Couches Minces, France.
Tsinghua Univ., China.
J Biomed Opt. 2019 Jul;24(7):1-9. doi: 10.1117/1.JBO.24.7.076004.
Mueller microscopy studies of fixed unstained histological cuts of human skin models were combined with an analysis of experimental data within the framework of differential Mueller matrix (MM) formalism. A custom-built Mueller polarimetric microscope was used in transmission configuration for the optical measurements of skin tissue model adjacent cuts of various nominal thicknesses (5 to 30 μm). The maps of both depolarization and polarization parameters were calculated from the corresponding microscopic MM images by applying a logarithmic Mueller matrix decomposition (LMMD) pixelwise. The parameters derived from LMMD of measured tissue cuts and the intensity of transmitted light were used for an automated segmentation of microscopy images to delineate dermal and epidermal layers. The quadratic dependence of depolarization parameters and linear dependence of polarization parameters on thickness, as predicted by the theory, was confirmed in our measurements. These findings pave the way toward digital histology with polarized light by presenting the combination of optimal optical markers, which allows mitigating the impact of tissue cut thickness fluctuations and increases the contrast of polarimetric images for tissue diagnostics.
对固定未经染色的人体皮肤模型组织学切片进行 Mueller 显微镜研究，并结合差分 Mueller 矩阵（MM）形式的实验数据分析。在传输配置中使用定制的 Mueller 偏振显微镜对不同标称厚度（5 至 30μm）的皮肤组织模型相邻切片进行光学测量。通过应用逐像素的对数 Mueller 矩阵分解（LMMD），从相应的微观 MM 图像中计算出退偏和偏振参数的图谱。从测量组织切片的 LMMD 得出的参数和透射光的强度用于显微镜图像的自动分割，以描绘真皮和表皮层。正如理论所预测的那样，退偏参数的二次依赖和偏振参数的线性依赖与厚度之间的关系在我们的测量中得到了证实。这些发现通过提出最佳光学标记的组合，为偏振光数字组织学铺平了道路，这可以减轻组织切片厚度波动的影响，并提高组织诊断的偏振图像对比度。
