计数切割：MAZTER-Seq 使用甲基化敏感的核糖核酸酶定量 mA 水平。
Counting the Cuts: MAZTER-Seq Quantifies mA Levels Using a Methylation-Sensitive Ribonuclease.
机构信息
Department of Molecular Biology, Science III, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
Department of Molecular Biology, Science III, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
出版信息
Cell. 2019 Jul 25;178(3):515-517. doi: 10.1016/j.cell.2019.07.006.
Garcia-Campos et al. describe MAZTER-seq, which deploys a sequence-specific, methylation-sensitive bacterial single-stranded ribonuclease MazF to provide nucleotide-resolution quantification of mA methylation sites. The study reveals many new sites and supports the idea of a predictable "mA code," where methylation levels are dictated primarily by local sequence at the site of methylation.
Garcia-Campos 等人描述了 MAZTER-seq，该方法利用序列特异性、甲基化敏感的细菌单链核糖核酸酶 MazF 提供核苷酸分辨率的 mA 甲基化位点定量。该研究揭示了许多新的位点，并支持了可预测的“mA 密码”的想法，即在该甲基化位点，甲基化水平主要由局部序列决定。
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