Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China; Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, China.
Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China.
J Pharm Biomed Anal. 2019 Oct 25;175:112779. doi: 10.1016/j.jpba.2019.112779. Epub 2019 Jul 17.
The combined usage of Ginseng Radix et Rhizoma (ginseng) and Ophiopogonis Radix is common in oriental countries for thousands of years. The major active constituents of ginseng are ginsenosides, and the conversion of ginsenosides to different metabolites by gut microbiota has been reported. However, the effect of Ophiopogonis Radix, especially its polysaccharides, on the metabolism of ginsenosides by gut microbiota is not known. Here, an in vitro metabolism of ginseng extract, or ginsenosides, in combination with or without Ophiopogon polysaccharide was conducted. A sensitive and reliable UPLC-MS/MS approach using multiple reaction monitoring (MRM) in positive ion mode was developed simultaneously to quantify 22 ginsenosides in the broth of gut microbiota. After fermentation with the microbiota, 15 ginsenosides were detected and quantified, including 6 primary ginsenosides, i.e. Rb, Rc, Rb, Rb, Rd and Re, and 9 metabolites, i.e. F, Rg, compound K, Rh, PPD, Rg, Rh, Rg and PPT. The quantitative results therefore revealed the elimination of primary ginsenosides and the formation of their metabolites in time-dependent manners. Furthermore, Ophiopogon polysaccharide was shown to stimulate the metabolism of ginsenosides, triggered by gut microbiota. Our study can be extended to investigate the metabolism of different Panax species by gut microbiota when combining with other herbs.
人参和麦冬在东方国家被联合使用已有数千年的历史。人参的主要活性成分是人参皂苷，其在肠道微生物群的作用下可转化为不同的代谢物。然而，麦冬，尤其是其多糖，对肠道微生物群中人参皂苷代谢的影响尚不清楚。本研究采用超高效液相色谱-串联质谱联用（UPLC-MS/MS），以正离子模式多反应监测（MRM）同时定量分析人参提取物或人参皂苷与或不与人参麦冬多糖组合后的代谢产物。建立了一种灵敏、可靠的方法，可同时定量分析肠道微生物菌群中肉汤中的 22 个人参皂苷。经微生物菌群发酵后，检测到并定量了 15 个人参皂苷，包括 6 种初级人参皂苷，即 Rb1、Rc、Rb2、Rb3、Rd 和 Re，以及 9 种代谢产物，即 F、Rg1、Rh2、Rg3、PPD、Rg2、Rh1、Rg5 和 PPT。定量结果表明，初级人参皂苷及其代谢物的消除呈时间依赖性。此外，麦冬多糖可刺激肠道微生物群代谢人参皂苷。当与其他草药结合时，我们的研究可以扩展到研究不同属植物的代谢情况。
