Xu Lei, Xu Jing, Shi Guohui, Xiao Shengnan, Dai Rongke, Wu Shao, Sun Baoshan, Zhang Xiaoshu, Zhao Yuqing
School of Functional Food and Wine, Shenyang Pharmaceutical University Shenyang 110016 China
Key Laboratory of Structure-based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University Shenyang 110016 China.
RSC Adv. 2020 Dec 11;10(72):44050-44057. doi: 10.1039/d0ra07517e. eCollection 2020 Dec 9.
In this paper, we used the flash extraction method (FEM) to extract ginsenosides from mountain cultivated ginseng (MCG), optimized the FEM process by response surface methodology (RSM), and separated 23 kinds of ginsenosides from MCG, including rare ginsenoside Rg, 20(/)-Rg, Rk, 20()-Rh, 20()-Rh, F and Rg. Among them, notoginsenoside R was isolated from MCG for the first time. Additionally, we established an HPLC-FT-ICR-MS method to accurately identify 20 ginsenosides in MCG, and quantitatively analyzed the differences in the content of rare ginsenosides in MCG and Garden-Cultivated Ginseng (CG) by HPLC-UV. The results showed that the chemical components of MCG and CG were similar, but the ginsenoside content of MCG was double that of CG. Notably, the content of ginsenoside 20 ()-Rh and 20 ()-Rh had the largest difference, and the content in MCG was 33 and 24 times higher than that in CG, respectively. Through quantitative analysis, we clarified the reason why the activity of MCG is stronger than that of CG, which provided a theoretical basis for clinical application and further research of MCG.
在本文中，我们采用快速萃取法（FEM）从山地栽培人参（MCG）中提取人参皂苷，通过响应面法（RSM）优化FEM工艺，并从MCG中分离出23种人参皂苷，包括稀有皂苷Rg、20（/）-Rg、Rk、20（）-Rh、20（）-Rh、F和Rg。其中，三七皂苷R首次从MCG中分离得到。此外，我们建立了一种HPLC-FT-ICR-MS方法来准确鉴定MCG中的20种人参皂苷，并通过HPLC-UV定量分析了MCG和园参（CG）中稀有皂苷含量的差异。结果表明，MCG和CG的化学成分相似，但MCG的人参皂苷含量是CG的两倍。值得注意的是，人参皂苷20（）-Rh和20（）-Rh的含量差异最大，MCG中的含量分别比CG高33倍和24倍。通过定量分析，我们阐明了MCG活性强于CG的原因，为MCG的临床应用和进一步研究提供了理论依据。
