Department of Biomedical Engineering, The University of Texas at Austin, Austin, USA; Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, USA; Department of Biomedical Engineering, Faculty of New Science and Technology, The University of Tehran, Iran.
Department of Biomedical Engineering, Faculty of New Science and Technology, The University of Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109860. doi: 10.1016/j.msec.2019.109860. Epub 2019 Jun 3.
This paper describes the design of stimuli-sensitive theranostic nanoparticles, composed of reduced graphene oxide (rGO) self-assembled on thermosensitive liposomes encapsulated doxorubicin (DOX) and carbon quantum dot (CQD) (CQD-DOX-rGO-Tlip). The rGO-Tlip particles have been observed to be flower-shaped objects. The thermoresponsive and theranostic potential of CQD-DOX-rGO-Tlips have been studied using differential scanning calorimetry (DSC), ultraviolet visible spectroscopy (UV-Vis), Raman spectroscopy and photoluminescent assays. The chemo-photothermal potential of rGO-Tlip on MD-MB-231 cells during NIR laser irradiation has been examined using MTT assay. Also, the ability of rGO-Tlip to be taken up by MD-MB-231 cells has been studied using confocal microscopy and flowcytometry. The results indicate that CQD-DOX-rGO-Tlips achieve a synergistic effect between photothermal therapy and chemotherapy for cancer treatment. Furthermore, online monitoring drug release is accomplished by studying the emission intensity of CQD while DOX released.
本文描述了刺激响应型治疗纳米粒子的设计，该粒子由负载多柔比星（DOX）和碳量子点（CQD）的热敏脂质体自组装的还原氧化石墨烯（rGO）（CQD-DOX-rGO-Tlip）组成。rGO-Tlip 颗粒呈花状。通过差示扫描量热法（DSC）、紫外可见分光光度法（UV-Vis）、拉曼光谱和光致发光法研究了 CQD-DOX-rGO-Tlips 的热响应和治疗潜力。在近红外激光照射下，通过 MTT 法研究了 rGO-Tlip 对 MD-MB-231 细胞的化学光热潜力。此外，通过共聚焦显微镜和流式细胞术研究了 rGO-Tlip 被 MD-MB-231 细胞摄取的能力。结果表明，CQD-DOX-rGO-Tlips 实现了光热治疗和化疗联合治疗癌症的协同作用。此外，通过研究 CQD 的发射强度来实现 DOX 释放的在线监测。
