Department of Medical Oncology, Xinxiang Central Hospital, The Fourth Clinical College of Xinxiang Medical University, Xinxiang, Henan, China.
Department of Inorganic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, India.
Drug Deliv. 2020 Dec;27(1):791-804. doi: 10.1080/10717544.2020.1765431.
The combined chemotherapy and photodynamic therapy have significant advantages for cancer treatments, which have higher therapeutic effects compared with other medicines. Herein, we focused on the synthesis of carbon quantum dot (CQD) based nanocarrier system. CQD and 5-aminolevulinic acid (5-ALA) were conjugated with mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin (CQD-Glu-β-CD) moiety, and finally, the anticancer chemotherapy doxorubicin (DOX) drug was loaded in the 5-ALA-CQD-Glu-β-CD system. The stepwise physicochemical changes for the preparation of the DOX loaded 5-ALA-CQD-Glu-β-CD system were investigated by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), atomic force microscopy (AFM), and Raman fluorescence spectroscopy. The encapsulation efficiency of DOX in 5-ALA-CQD-Glu-β-CD was observed at ∼83.0%, and the loading capacity of DOX is ∼20.37%. The releasing of DOX and 5-ALA was observed through the UV-vis spectroscopy by the value of 487 nm and 253 nm, respectively. By the investigation against the breast MCF-7 cancer cells, the high cytotoxicity and morphological changes of cancer cells were observed by the treating of DOX/5-ALA-CQD-Glu-β-CD. The generation of reactive oxygen species (ROS) upon 635 nm (25 mW cm) for 15 min laser irradiation-induced improved the therapeutic effects. cellular uptake studies recommend the synthesized DOX/5-ALA-CQD-Glu-β-CD nanocarrier could significantly enhance the cell apoptosis and assist in the MCF-7 cell damages. The result suggests a multifunctional therapeutic system for chemo/photodynamic synergistic effects on cancer therapy.
联合化疗和光动力疗法在癌症治疗方面具有显著优势，与其他药物相比，其治疗效果更高。在此，我们专注于基于碳量子点（CQD）的纳米载体系统的合成。CQD 和 5-氨基酮戊酸（5-ALA）与单（5-BOC-保护-谷氨酰胺-6-脱氧）β-环糊精（CQD-Glu-β-CD）部分连接，最后将抗癌化疗药物阿霉素（DOX）载入 5-ALA-CQD-Glu-β-CD 系统中。通过傅里叶变换红外（FT-IR）光谱、X 射线衍射（XRD）、透射电子显微镜（TEM）、原子力显微镜（AFM）和拉曼荧光光谱研究了 DOX 负载 5-ALA-CQD-Glu-β-CD 系统制备过程中的分步物理化学变化。观察到 DOX 在 5-ALA-CQD-Glu-β-CD 中的包封效率约为 83.0%，DOX 的载药量约为 20.37%。通过 UV-vis 光谱观察到 DOX 和 5-ALA 的释放，分别通过 487nm 和 253nm 的 值观察到。通过对乳腺癌 MCF-7 癌细胞的研究，通过用 DOX/5-ALA-CQD-Glu-β-CD 处理观察到癌细胞的高细胞毒性和形态变化。635nm（25mW cm）激光照射诱导下活性氧（ROS）的产生提高了治疗效果。细胞摄取研究表明，合成的 DOX/5-ALA-CQD-Glu-β-CD 纳米载体可以显著增强细胞凋亡，并有助于 MCF-7 细胞损伤。结果表明，该多功能治疗系统可协同化疗/光动力疗法用于癌症治疗。
