Department of Analytical Chemistry, Universidad Autónoma de Nuevo León School of Medicine, Av Dr. Aguirre Pequeño and Madero S/N, Mitras Centro, C.P 64460, Monterrey, Nuevo León, Mexico.
Department of Internal Medicine, Universidad Autónoma de Nuevo León, "Dr. José E. González" University Hospital Liver Unit, Av. Gonzalitos 235, Mitras Centro, C.P. 64460, Monterrey, Nuevo León, Mexico.
BMC Complement Altern Med. 2019 Jul 26;19(1):186. doi: 10.1186/s12906-019-2604-7.
Oxidative stress and the inflammatory process are involved in ischemia-reperfusion (I/R) injury. Juglans mollis has been reported as having antioxidant activity, which could attenuate the damage caused by I/R. We evaluated whether a methanolic extract of Juglans mollis (JM) exhibits nephroprotective activity in a Wistar rat model of I/R injury.
Four groups of six rats were used: Sham, I/R, JM, and JM + I/R. Two groups were dosed with JM (300 mg/kg) for 7 days before I/R. I/R injury was induced by clamping the renal hilums for 45 min and then reperfusing the kidneys for 15 h. Blood samples were taken to evaluate the levels of alanine aminotransferase (ALT), blood urea nitrogen, creatinine, superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor α (TNF-α).
The levels of creatinine, ALT, MDA, IL-1β, IL-6, and TNF-α were lower in JM + I/R than in I/R rats, whereas SOD level only was higher in JM + I/R than in Sham rats. No biochemical or histological damage was observed in JM rats compared with Sham rats; however, less histological damage was observed in JM + I/R rats compared with I/R rats.
To our knowledge, this is the first report of nephroprotective activity of J. mollis against damage induced by I/R. This activity may be related to decreased levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and modulation of oxidative stress markers (SOD and MDA) observed in the present study.
氧化应激和炎症过程参与了缺血再灌注（I/R）损伤。已报道胡桃楸具有抗氧化活性，可减轻 I/R 引起的损伤。我们评估了胡桃楸甲醇提取物（JM）在 Wistar 大鼠 I/R 损伤模型中是否具有肾保护活性。
使用 6 只大鼠的 4 组：假手术、I/R、JM 和 JM+I/R。两组在 I/R 前 7 天用 JM（300mg/kg）给药。I/R 损伤通过夹闭肾门 45 分钟，然后再灌注肾脏 15 小时来诱导。采集血液样本以评估丙氨酸氨基转移酶（ALT）、血尿素氮、肌酐、超氧化物歧化酶（SOD）、丙二醛（MDA）、白细胞介素 1β（IL-1β）、IL-6 和肿瘤坏死因子 α（TNF-α）的水平。
JM+I/R 组的肌酐、ALT、MDA、IL-1β、IL-6 和 TNF-α水平低于 I/R 组，而 SOD 水平仅高于 Sham 组。与 Sham 组相比，JM 组未观察到生化或组织学损伤；然而，与 I/R 组相比，JM+I/R 组观察到的组织学损伤较少。
据我们所知，这是首次报道胡桃楸对 I/R 引起的损伤具有肾保护活性。这种活性可能与本研究中观察到的促炎细胞因子（IL-1β、IL-6 和 TNF-α）水平降低和氧化应激标志物（SOD 和 MDA）的调节有关。
