Tantengco Ourlad Alzeus G, Nakura Yukiko, Yoshimura Michinobu, Llamas-Clark Erlidia F, Yanagihara Itaru
College of Medicine, University of the Philippines Manila, Manila, Philippines.
Department of Developmental Medicine, Research Institute, Osaka Women's and Children's Hospital, Izumi, Osaka, Japan.
Asian Pac J Cancer Prev. 2019 Jul 1;20(7):2103-2107. doi: 10.31557/APJCP.2019.20.7.2103.
Background: PIK3CA and MDM2 SNP309 have been studied to be associated with cervical cancer. PIK3CA mutation is associated with poor treatment response and low survival rate while MDM2 is associated with tumorigenesis and poor prognosis in cervical cancer. Thus, we determined the prevalence of PIK3CA and MDM2 mutations in Filipino cervical cancer patients. Methods: Twenty-eight formalin-fixed paraffin-embedded cervical squamous cell carcinoma and 16 non-malignant cervix tissue biopsies of Filipino patients were subjected to PIK3CA gene and MDM2 SNP309 (rs2279744) analysis. Results: PIK3CA gene was found mutated in three (10.71 %) out of 28 cervical cancer patients included in this study. Among the HPV-negative cervical cancer patients, two (28.57 %) were positive for PIK3CA mutation and only one (4.76 %) tested positive among the HPV-positive cervical cancer patients. MDM2 SNP309 analysis revealed that T allele (71.43%) was more common in cervical cancer patients compared to the control group. TG genotype (p = 0.03; OR = 0.18, 95% CI 0.04-0.76) was associated with lower rates of cervical cancer when TT genotype was used as a reference point. Conclusion: PIK3CA gene mutation was present among Filipino cervical cancer patients and not in control patients. MDM2 SNP309 analysis revealed that TG genotype has lower association to cervical cancer when compared with the TT and GG genotypes.
已有研究表明PIK3CA和MDM2 SNP309与宫颈癌有关。PIK3CA突变与治疗反应差和生存率低相关，而MDM2与宫颈癌的肿瘤发生和预后不良相关。因此，我们确定了菲律宾宫颈癌患者中PIK3CA和MDM2突变的发生率。方法：对28例菲律宾患者的福尔马林固定石蜡包埋宫颈鳞状细胞癌和16例非恶性宫颈组织活检标本进行PIK3CA基因和MDM2 SNP309（rs2279744）分析。结果：本研究纳入的28例宫颈癌患者中，有3例（10.71%）发现PIK3CA基因发生突变。在HPV阴性的宫颈癌患者中，2例（28.57%）PIK3CA突变呈阳性，而在HPV阳性的宫颈癌患者中只有1例（4.76%）检测呈阳性。MDM2 SNP309分析显示，与对照组相比，T等位基因（71.43%）在宫颈癌患者中更常见。以TT基因型为参照点时，TG基因型（p = 0.03；OR = 0.18，95%CI 0.04 - 0.76）与宫颈癌发生率较低相关。结论：菲律宾宫颈癌患者中存在PIK3CA基因突变，而对照患者中未发现。MDM2 SNP309分析显示，与TT和GG基因型相比，TG基因型与宫颈癌的关联较低。
