用豌豆凝集素检测到的形态不佳但顶体完整正常的人类精子比例与体外受精相关。

The proportion of human sperm with poor morphology but normal intact acrosomes detected with Pisum sativum agglutinin correlates with fertilization in vitro.

作者信息

Liu D Y, Baker H W

机构信息

Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, Australia.

出版信息

Fertil Steril. 1988 Aug;50(2):288-93. doi: 10.1016/s0015-0282(16)60075-4.

DOI:10.1016/s0015-0282(16)60075-4

PMID:3135205

Abstract

Sperm acrosomes were examined with fluorescein-labeled pisum sativum agglutinin in 137 in vitro fertilization treatments. The percentage of sperm with normal intact acrosomes in semen was correlated with sperm concentration, morphology, viability, and motility. The proportion of sperm with normal intact acrosomes was significantly increased by the "swim up" selection from semen. Fertilization rates in the whole group studied were significantly related to percentage normal morphology, concentration of sperm inseminated and cause of infertility. However, with normal morphology less than 30%, the proportion of sperm with normal intact acrosomes in the insemination medium was the only significant factor related to fertilization rate in the logistic regression analysis. Thus, assessment of acrosomes may be of clinical value for predicting the fertility of men with poor sperm morphology.

摘要

在137例体外受精治疗中，使用荧光素标记的豌豆凝集素对精子顶体进行了检测。精液中顶体正常完整的精子百分比与精子浓度、形态、活力和运动能力相关。通过精液“上游”筛选，顶体正常完整的精子比例显著增加。在整个研究组中，受精率与正常形态百分比、授精精子浓度和不孕原因显著相关。然而，当正常形态低于30%时，在逻辑回归分析中，授精介质中顶体正常完整的精子比例是与受精率相关的唯一显著因素。因此，评估顶体对于预测精子形态不佳男性的生育能力可能具有临床价值。