School of Science, Monash University Malaysia, Subang Jaya, Malaysia.
School of Medicine and Health Sciences, Monash University Malaysia, Subang Jaya, Malaysia.
J Food Biochem. 2019 Feb;43(2):e12717. doi: 10.1111/jfbc.12717. Epub 2018 Nov 4.
The present study aimed to outline the physiological and metabolic disparity between chow- and purified ingredient-based high-fat diets and their efficacy in the induction of metabolic syndrome (MetS). Male, 3-week-old Sprague Dawley rats were randomly assigned to chow-based control diet, chow-based high-fat diet, purified control diet, and purified high-fat diet for 12 weeks. Physical and biochemical changes were documented. Chow-based diets, irrespective of the lipid content, resulted in significantly lower weight gain and organ weight compared to purified ingredient-based diets. Circulating insulin, total proteins, albumin, and certain lipid components like the triglycerides, total cholesterol, and high-density lipoprotein-cholesterol were also lower in the chow-based diet groups. Both chow- and purified high-fat diets induced central obesity, hypertension, and hyperglycaemia, but the latter was associated with earlier onset of the metabolic aberrations and additionally, dyslipidaemia. In conclusion, purified high-fat diet is a better diet for MetS induction in rats. PRACTICAL APPLICATIONS: Modeling metabolic syndrome is commonly accomplished with the use of chow- or purified ingredient diets enriched with carbohydrates and/or lipids, but the differences and associated drawbacks are unclear. This study highlights that chow- or modified chow-based diets have a tendency to introduce unwanted metabolic changes which are inconsistent with the progression of metabolic syndrome. Thus, the use of these diets in metabolic disease study should be avoided. On the other hand, purified high-fat diet which can effectively induce the features of metabolic syndrome is highly recommended.
本研究旨在概述基于标准饲料和纯化成分的高脂肪饮食之间的生理和代谢差异，以及它们在诱导代谢综合征（MetS）方面的效果。雄性，3 周龄的 Sprague Dawley 大鼠被随机分配到标准饲料对照组、标准饲料高脂肪组、纯化成分对照组和纯化成分高脂肪组，进行 12 周的喂养。记录了身体和生化变化。与基于纯化成分的饮食相比，标准饲料饮食，无论其脂肪含量如何，导致体重增加和器官重量显著降低。循环胰岛素、总蛋白、白蛋白和某些脂质成分，如甘油三酯、总胆固醇和高密度脂蛋白胆固醇，在标准饲料饮食组中也较低。标准饲料和纯化成分高脂肪饮食都诱导了中心性肥胖、高血压和高血糖，但后者与代谢异常的早期发生有关，此外还伴有血脂异常。总之，纯化成分高脂肪饮食是诱导大鼠代谢综合征的更好饮食。实际应用：代谢综合征的建模通常使用富含碳水化合物和/或脂肪的标准饲料或纯化成分饲料来完成，但差异和相关缺点尚不清楚。本研究表明，标准饲料或改良标准饲料喂养有引入与代谢综合征进展不一致的非期望代谢变化的趋势。因此，应避免在代谢性疾病研究中使用这些饮食。另一方面，建议使用能够有效诱导代谢综合征特征的纯化成分高脂肪饮食。
