School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Biomater Sci. 2019 Oct 1;7(10):4075-4087. doi: 10.1039/c9bm00862d. Epub 2019 Jul 29.
Titanium (Ti) is a widely used implant material in clinics; however, failures still frequently occur due to its bioinertness and poor antibacterial capability. Post-implant infections most likely occur within the first two weeks. Thereafter, the host immune system lowers the infection risk, and biosafety becomes the first consideration. Therefore, endowing biomedical Ti with a time-dependent bactericidal effect is of considerable interest. In this study, Ag nanoparticles (NPs) as the antibacterial agent were incorporated deeply into TiO nanotubes prepared on the sandblasted and etched (SLA) Ti surface. The incorporated Ag NPs were verified to automatically transform from a free state to an immobilized state, rendering the constructed platform exhibit a self-adjusting antibacterial effect. It showed strong "release bactericidal" activity in the early phase that gradually changed to the "contact bactericidal" ability. Such a smart alteration could satisfy the varied antibacterial requirements in different periods after biomaterial implantation. Moreover, the nanotubular structure could accelerate apatite formation and improve cell adhesion and proliferation when compared with those of commercially used SLA implants. Based on these results, it can be concluded that Ag-NP-incorporated micro-nanostructured Ti has worthwhile biological and time-dependent antibacterial properties, and it can have promising applications in orthopedics, dentistry, and fabrication of other biomedical devices.
钛（Ti）是临床中广泛使用的植入材料；然而，由于其生物惰性和抗菌能力差，仍然经常发生失效。植入后感染很可能在最初的两周内发生。此后，宿主的免疫系统降低了感染的风险，生物安全性成为首要考虑因素。因此，赋予生物医学 Ti 具有时间依赖性杀菌效果是非常有意义的。在这项研究中，将作为抗菌剂的 Ag 纳米颗粒（NPs）掺入到喷砂酸蚀（SLA）Ti 表面制备的 TiO 纳米管中。证实掺入的 Ag NPs 自动从自由状态转变为固定状态，从而使构建的平台表现出自适应的抗菌效果。它在早期表现出强烈的“释放杀菌”活性，逐渐转变为“接触杀菌”能力。这种智能变化可以满足生物材料植入后不同时期的不同抗菌要求。此外，与商用 SLA 植入物相比，纳米管状结构可以加速磷灰石的形成，提高细胞的粘附和增殖。基于这些结果，可以得出结论，Ag-NP 掺入的微纳结构 Ti 具有有价值的生物和时间依赖性抗菌特性，并且在骨科、牙科和其他生物医学设备的制造方面具有广阔的应用前景。
