Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, People's Republic of China.
Microsc Res Tech. 2012 Jan;75(1):54-64. doi: 10.1002/jemt.21024. Epub 2011 May 26.
Light and polarized microscope was applied to authenticate 32 Cordyceps and 6 artificial counterfeits and 8 fermented Cordyceps as well as 7 Cordyceps capsules available in Hong Kong markets. Results showed that transverse sections of stroma and powder of larvae can be used to differentiate C. sinensis from its counterfeits. The fermented Cordyceps are in powder form. Among the eight fermented Cordyceps collected, half of them were pure; three were a mixture of fermented Cordyceps and soya beans; one was a mixture of unknown plant tissues and soya beans. For the seven Cordyceps capsules, the powders of five samples were a mixture of fermented Cordyceps and soya beans; the powders of other two were a mixture of C. sinensis stroma powder and fermented Cordyceps. The study indicated that the microscopy is an unambiguous method that requests fewer sample for the authentication of valuable Chinese medicine-C. sinensis and its related products.
应用光学显微镜和偏光显微镜对 32 株天然冬虫夏草及其 6 种伪品、8 株发酵冬虫夏草以及 7 种市售冬虫夏草胶囊进行鉴别。结果表明，横切面的虫体和幼虫粉可以将冬虫夏草与其伪品区分开来。发酵冬虫夏草呈粉末状。在所采集的 8 株发酵冬虫夏草中，有一半是纯发酵冬虫夏草，有 3 株是发酵冬虫夏草与黄豆的混合物，有 1 株是未知植物组织与黄豆的混合物。对于 7 种冬虫夏草胶囊，其中 5 种粉末是发酵冬虫夏草与黄豆的混合物，另外 2 种粉末是冬虫夏草横切面粉末与发酵冬虫夏草的混合物。该研究表明，显微镜检查法是一种快速、简单且明确的方法，仅需少量样本即可对贵重中药——冬虫夏草及其相关产品进行鉴别。
