Chen Chang-Qing, Yan Dong, Jiang Yun, Xu Peng, Chu Yi-Xuan, Gao Jie
College of Agronomy,Jilin Agricultural University Changchun 130118,China.
College of Life Science,Jilin Agricultural University Changchun 130118,China.
Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):2015-2019. doi: 10.19540/j.cnki.cjcmm.20190329.101.
This study was aimed to clarify the toxicity indoor and inhibition effect of biocontrol strain NJ13 and its mixture with chemical fungicides against Fusarium solani causing ginseng root rot. The method of mycelial growth rate and Sun Yunpei method were used to determine the indoor toxicity and co-toxicity coefficient of strain NJ13 and their mixture with chemical pesticides against F. solani. The dual culture assay method,mixed culture method and microscopic observation were used to determine the sporulation and germination of spores and mycelial growth and morphological change of hyphae of F. solani treated by strain NJ13. The results of toxicity indoor showed that strain NJ13 had the best inhibitory effect on pathogen,and its EC_(50) value was 0. 071 mg·L~(-1). It was all synergistic for antifungal effect that strain NJ13 was mixed with propiconazole and difenoconazole respectively with a range from 1 ∶4 to 4 ∶1( volume ratio). Both of optimal ratios were 1 ∶1,and the co-toxicity coefficients were 848. 70 and 859. 73,respectively. The strain NJ13 could inhibit the sporulation,germination and mycelial growth of F. solani. The biocontrol strain NJ13 had an inhibition effect on F. solani,and the optimal antifungal ratio of strain NJ13 mixed with propiconazole and difenoconazole was obtained.
本研究旨在阐明生防菌株NJ13及其与化学杀菌剂的混合物对引起人参根腐病的茄腐镰刀菌的室内毒性和抑制效果。采用菌丝生长速率法和孙云沛法测定菌株NJ13及其与化学农药的混合物对茄腐镰刀菌的室内毒性和共毒系数。采用对峙培养法、混合培养法和显微镜观察法测定菌株NJ13处理后茄腐镰刀菌的孢子形成、孢子萌发、菌丝生长及菌丝形态变化。室内毒性结果表明，菌株NJ13对病原菌的抑制效果最佳，其EC50值为0.071 mg·L-1。菌株NJ13与丙环唑和苯醚甲环唑分别按1∶4至4∶1（体积比）混合时，对真菌的抑制作用均表现为增效作用。最佳比例均为1∶1，共毒系数分别为848.70和859.73。菌株NJ13可抑制茄腐镰刀菌的孢子形成、萌发和菌丝生长。生防菌株NJ13对茄腐镰刀菌有抑制作用，并得出了菌株NJ13与丙环唑和苯醚甲环唑混合的最佳抑菌比例。
