Wang Caicheng, Ye Xiujuan, Ng Tzi Bun, Zhang Wenjing
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
J Agric Food Chem. 2021 Feb 24;69(7):2051-2061. doi: 10.1021/acs.jafc.0c06106. Epub 2021 Feb 11.
A bacterium identified as with a growth inhibitory effect against , a pathogen that caused basal stem rot in the passion fruit , was isolated in this study. From the fermentation broth of , a type of antifungal peptide (named BVAP) with a molecular weight of ca. 1.5 kDa was purified and found to be fengycin. BVAP suppressed mycelial growth in with an IC of 5.58 μg/mL, which was superior to those of the chemical fungicides thiram (41.24 μg/mL) and hymexazol (343.31 μg/mL). The antifungal activity remained stable after exposure to 50-100 °C or following incubation with solutions at pH 1-3. Further research revealed that BVAP increased the permeability of the mycelial membrane, brought about swelling at the tips of hyphae, and elicited abnormal accumulation of nucleic acids and chitin at the sites of swelling. These findings indicate that BVAP possessed a remarkable biocontrol potential toward .
在本研究中，分离出了一种对引起百香果基部茎腐病的病原菌具有生长抑制作用的细菌。从该细菌的发酵液中，纯化出了一种分子量约为1.5 kDa的抗真菌肽（命名为BVAP），发现其为丰原素。BVAP抑制病原菌菌丝生长的IC50为5.58 μg/mL，优于化学杀菌剂福美双（41.24 μg/mL）和噁霉灵（343.31 μg/mL）。在50 - 100 °C下处理或在pH 1 - 3的溶液中孵育后，其抗真菌活性保持稳定。进一步研究表明，BVAP增加了病原菌菌丝膜的通透性，导致菌丝尖端肿胀，并在肿胀部位引发核酸和几丁质的异常积累。这些发现表明，BVAP对该病原菌具有显著的生物防治潜力。
