College of Plant Protection, Jilin Agricultural University, Changchun, Jilin, China.
J Appl Microbiol. 2021 Jan;130(1):165-178. doi: 10.1111/jam.14771. Epub 2020 Jul 22.
We aimed to purify an antimicrobial protein from Bacillus amyloliquefaciens FS6 culture supernatant, verify its antimicrobial activity against Fusarium solani and evaluate its biocontrol potential for ginseng root rot.
The antimicrobial protein was purified from FS6 culture supernatant using ammonium sulphate precipitation, anion exchange and gel chromatography. Based on mass spectrometry results, the purified protein was identified as an antimicrobial protein of the LCI family and was designated APC . The APC recombinant protein expressed in Escherichia coli (BL21) significantly inhibited F. solani and decreased the infection and spread of F. solani in ginseng root. An overexpressing APC strain FS6-APC was constructed and shown to have enhanced antimicrobial activity compared to the wild-type strain FS6.
The APC protein shows strong antimicrobial activity against F. solani, reduces the incidence and severity of ginseng root rot caused by F. solani and exhibits a great biocontrol potential.
This study reports the inhibitory activity of APC protein (LCI family) against F. solani and its protective efficacy on ginseng root rot. These findings provide a scientific basis for future research on the biocontrol mechanism, as well as the development and application of FS6.
从解淀粉芽孢杆菌 FS6 培养上清液中纯化一种抗菌蛋白，验证其对茄病镰刀菌的抗菌活性，并评估其对人参根腐病的生防潜力。
采用硫酸铵沉淀、阴离子交换和凝胶层析从 FS6 培养上清液中纯化抗菌蛋白。根据质谱结果，鉴定纯化的蛋白为 LCI 家族的抗菌蛋白，并将其命名为 APC。在大肠杆菌（BL21）中表达的 APC 重组蛋白显著抑制了茄病镰刀菌，并降低了人参根中茄病镰刀菌的感染和扩散。构建了 APC 过表达菌株 FS6-APC，并显示出比野生型菌株 FS6 更强的抗菌活性。
APC 蛋白对茄病镰刀菌表现出强烈的抗菌活性，降低了由茄病镰刀菌引起的人参根腐病的发病率和严重程度，具有很大的生防潜力。
本研究报道了 APC 蛋白（LCI 家族）对茄病镰刀菌的抑制活性及其对人参根腐病的保护效果。这些发现为 FS6 的生防机制研究以及开发应用提供了科学依据。
