Present address: R&D Center, BioPS Co., Ltd., Daedeuk Valley Campus, Hannam University, Daejon 34054, Republic of Korea.
Faculty of Science Education, Jeju National University, Jeju 63243, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3287-3292. doi: 10.1099/ijsem.0.003625. Epub 2019 Jul 29.
The taxonomic status of a Gram-reaction-negative, aerobic, motile, rod-shaped bacterium, designated strain GH3-15, was examined by a polyphasic approach. The strain, which was isolated from the rhizosphere mudflat of a halophyte at the seashore of Gangwha Island, Republic of Korea, was found to belong to the family based on 16S rRNA gene sequences. The closest phylogenetic neighbour was SM1501 (98.3 % sequence similarity). Levels of 16S rRNA gene sequence similarity of strain GH3-15 to other members of the family were <97.1 %. The respiratory quinone was Q-10. The major fatty acids were Cω6, Cω7, C 2-OH, 11-methyl Cω7 and C. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The novel isolate exhibited growth at 20-40 °C, at pH 5-9, and in the presence of 1-7 % (w/v) NaCl. DNA relatedness between strain GH3-15 and its closet relative was 32.9±8.8 %. On the basis of phenotypic, chemotaxonomic and DNA-DNA hybridization data, in addition to a distinct phylogenetic position, strain GH3-15 (=KCTC 62380=JCM 32445) represents a novel species of the genus for which the name sp. nov. is proposed.
一株革兰氏反应阴性、需氧、能动、杆状细菌的分类地位采用多相分类法进行了研究。该菌株从韩国 Gangwha 岛海滨盐生植物根际泥滩中分离得到，属于 科。基于 16S rRNA 基因序列，其最密切的系统发育近邻为 SM1501（相似度为 98.3%）。与 科其他成员的 16S rRNA 基因序列相似度<97.1%。呼吸醌为 Q-10。主要脂肪酸为 Cω6、Cω7、C 2-OH、11-甲基 Cω7 和 C。极性脂为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和神经酰胺糖脂。新分离株在 20-40°C、pH5-9 和 1-7%（w/v）NaCl 存在下生长。GH3-15 菌株与其最接近的亲缘菌株之间的 DNA 相关性为 32.9±8.8%。基于表型、化学分类和 DNA-DNA 杂交数据，以及独特的系统发育位置，菌株 GH3-15（=KCTC 62380=JCM 32445）代表了一个新的属种，建议将其命名为 sp. nov.。
