Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1470-1477. doi: 10.1099/ijsem.0.003824.
A Gram-staining-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated as JBTF-M21, was isolated from a tidal flat sediment on the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences indicated that JBTF-M21 fell within the clade comprising the type strains of species of the genus . JBTF-M21 exhibited 16S rRNA gene sequence similarities of 97.0-98.4 % to the type strains of , , , , , and and 93.7-96.6 % to the type strains of the other species of the genus . The ANI and dDDH values between JBTF-M21 and the type strains of , , and were 70.83-72.93 % and 18.0-18.8 %, respectively. Mean DNA-DNA relatedness values between JBTF-M21 and the type strains of , and were 12-24 %. The DNA G+C content of JBTF-M21 was 57.0 mol%. JBTF-M21 contained Q-10 as the predominant ubiquinone and Cω7 and Cω6 as the major fatty acids. The major polar lipids ofJBTF-M21 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. Distinguishing phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that JBTF-M21 is separated from species of the genus with validly published names. On the basis of the data presented, strain JBTF-M21 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JBTF-M21 (=KACC 19864=NBRC 113584).
一株革兰氏阴性、需氧、非运动、球形、卵形或杆状细菌菌株，被命名为 JBTF-M21，从韩国黄海潮滩沉积物中分离得到。基于 16S rRNA 基因序列的系统发育邻接法树表明，JBTF-M21 属于包含属内各菌种的聚类分支。JBTF-M21 与属内各菌种的模式菌株的 16S rRNA 基因序列相似性为 97.0-98.4%，与属内其他菌种的模式菌株的 16S rRNA 基因序列相似性为 93.7-96.6%。JBTF-M21 与模式菌株之间的 ANI 和 dDDH 值分别为 70.83-72.93%和 18.0-18.8%。JBTF-M21 与模式菌株之间的 DNA-DNA 相关度值分别为 12-24%。JBTF-M21 含有 Q-10 作为主要的泛醌，Cω7 和 Cω6 作为主要的脂肪酸。JBTF-M21 的主要极性脂类为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和神经酰胺糖脂。表型特征、系统发育和遗传特性的区别表明，JBTF-M21 与已发表名称的属内各菌种明显不同。根据所提供的数据，菌株 JBTF-M21 被认为代表了一个属的新种，建议将其命名为 sp. nov.。其模式菌株为 JBTF-M21（=KACC 19864=NBRC 113584）。
