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一种存在于欧洲卵菌纲菌 Plasmopara viticola 分离物中的分泌型 WY 结构域蛋白可诱导葡萄和烟草物种的细胞死亡。

A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species.

机构信息

SVQV, Université de Strasbourg, INRA, Colmar, France.

University of Cambridge, Sainsbury Laboratory (SLCU), Cambridge, United Kingdom.

出版信息

PLoS One. 2019 Jul 29;14(7):e0220184. doi: 10.1371/journal.pone.0220184. eCollection 2019.

DOI:10.1371/journal.pone.0220184
PMID:31356604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6663016/
Abstract

Plasmopara viticola is a biotrophic oomycete pathogen causing grapevine downy mildew. We characterized the repertoire of P. viticola effector proteins which may be translocated into plants to support the disease. We found several secreted proteins that contain canonical dEER motifs and conserved WY-domains but lack the characteristic RXLR motif reported previously from oomycete effectors. We cloned four candidates and showed that one of them, Pv33, induces plant cell death in grapevine and Nicotiana species. This activity is dependent on the nuclear localization of the protein. Sequence similar effectors were present in seven European, but in none of the tested American isolates. Together our work contributes a new type of conserved P. viticola effector candidates.

摘要

葡萄霜霉病菌是一种专性活体营养型卵菌病原体，可引起葡萄霜霉病。我们对其效应蛋白进行了特征分析，这些蛋白可能被转运到植物体内以辅助疾病发生。我们发现了一些分泌蛋白，它们含有典型的 dEER 基序和保守的 WY 结构域，但缺乏先前报道的卵菌效应物中特有的 RXLR 基序。我们克隆了其中的 4 个候选基因，并证实其中一个，Pv33，可诱导葡萄和烟草属植物的细胞死亡。这种活性依赖于蛋白的核定位。在来自欧洲的 7 个分离株中存在与序列相似的效应物，但在测试的美国分离株中均不存在。总的来说，我们的工作提供了一类新的保守的葡萄霜霉病菌效应子候选蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c46/6663016/fec1b216ecfa/pone.0220184.g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c46/6663016/4bc4f47cf4e1/pone.0220184.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c46/6663016/fec1b216ecfa/pone.0220184.g008.jpg

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