Institute of Agriculture and Tourism, K. Huguesa 8, 52440 Poreč, Croatia; Centre of Excellence for Biodiversity and Molecular Plant Breeding, Svetošimunska 25, 10000 Zagreb, Croatia.
Institute of Agriculture and Tourism, K. Huguesa 8, 52440 Poreč, Croatia.
Food Chem. 2019 Dec 1;300:125251. doi: 10.1016/j.foodchem.2019.125251. Epub 2019 Jul 24.
Targeted ultra-performance liquid chromatography with triple quadrupole mass spectrometric (UPLC-QqQ-MS/MS) profiling of phenolic compounds was utilised for varietal differentiation of 173 wines made from four red and six white grape varieties. Among 58 identified phenols many were found relevant as exclusive or partial discriminators between wines. Successful differentiation models were built by linear discriminant analysis with the percentage of correct classification higher than 95% in all cases, with peonidin 3-(6″-acetyl)-glucoside and taxifolin as the most potent differentiators between red, and cis-piceid between white monovarietal wines. Diverse typical colour attributes among the monovarietal wines were tentatively ascribed to the variations in the composition of monomeric anthocyanins. Plavac mali red wine exhibited the most specific composition, and its most typical samples were distinguished by the abundance in trans-fertaric acid, isorhapontin, phlorizin, quercetin 3-rhamnoside, and myricitrin. Despite positive correlations with particular astringent flavonols, the typical astringency of Plavac mali wine remained unresolved.
采用靶向超高效液相色谱-三重四极杆串联质谱（UPLC-QqQ-MS/MS）分析方法对来自 4 个红葡萄品种和 6 个白葡萄品种的 173 种葡萄酒中的酚类化合物进行了分析，以实现品种区分。在鉴定出的 58 种酚类化合物中，许多都被认为是葡萄酒之间的唯一或部分区分标志物。通过线性判别分析建立了成功的区分模型，在所有情况下，正确分类的百分比均高于 95%，其中矢车菊素 3-（6″-乙酰基）-葡萄糖苷和松脂素是区分红葡萄酒的最有效标志物，顺式白皮杉醇是区分白葡萄酒的最有效标志物。单品种葡萄酒之间存在的各种典型颜色特征，可能归因于单体花色苷组成的变化。普拉瓦茨马里红葡萄酒表现出最独特的组成，其最典型的样品通过反式酒石酸、异哈朴醇、根皮苷、槲皮素 3-鼠李糖苷和杨梅素的丰度来区分。尽管与特定涩味黄酮醇呈正相关，但普拉瓦茨马里葡萄酒的典型涩味仍未得到解决。
