Toxicology Laboratory, Center for Studies of Worker's Health and Human Ecology (CESTEH), National School of Public Health Sergio Arouca (ENSP), Oswaldo Cruz Foundation (Fiocruz), Leopoldo Bulhões Street, 1480, Manguinhos, Rio de Janeiro-RJ, CEP 21041-210, Brazil.
Department of Endemics Samuel Pessoa (DENSP), National School of Public Health Sergio Arouca (ENSP), Oswaldo Cruz Foundation (Fiocruz), Leopoldo Bulhões Street, 1480, Manguinhos, Rio de Janeiro-RJ, CEP 21041-210, Brazil.
Int J Environ Res Public Health. 2019 Jul 27;16(15):2682. doi: 10.3390/ijerph16152682.
Oxidative stress can be induced by mercury (Hg) exposure, including through fish consumption (diet), leading to health risks. The objective of this study was to evaluate the association between oxidative stress biomarkers and dietary Hg exposure levels in riverine children and adoluiaescents at Madeira River (RO/Brazil). Population from three riverine local communities presenting different fish consumption frequencies was sampled. Hg was determined in blood (ICP-MS) and glutathione (GSH); glutathione S-transferases (GST) and malondialdehyde (MDA) were determined in serum (spectrophotometry). Statistical analyses were performed using parametric and non-parametric tests. Multiple linear regression models and generalized additives models were also used to estimate the relationships between oxidative stress biomarkers and blood Hg. The juvenile riverine population from Cuniã RESEX presented the highest levels of oxidative stress and Hg levels in blood (GST = 27.2 (4.93) U/L, MDA = 1.69 (0.27) µmol/L, Hg = 20.6 (18.0) µg/L). This population also presented the highest frequency of fish consumption. The positive relation between Hg and GST and MDA, adjusted for individual characteristics, suggests an oxidative effect. This study shows the importance of oxidative stress biomarkers in the evaluation of dietary Hg exposure since initial and reversible metabolic changes were observed, enriching health risk assessments.
氧化应激可由汞（Hg）暴露引起，包括通过食用鱼类（饮食），从而带来健康风险。本研究的目的是评估马德拉河（巴西/罗赖马州）流域儿童和青少年的氧化应激生物标志物与膳食 Hg 暴露水平之间的关系。在三个具有不同鱼类食用频率的河流社区中抽取了人群样本。采用电感耦合等离子体质谱法（ICP-MS）测定血液中的 Hg，采用分光光度法测定血清中的谷胱甘肽（GSH）、谷胱甘肽 S-转移酶（GST）和丙二醛（MDA）。采用参数和非参数检验进行统计分析。还使用多元线性回归模型和广义添加剂模型来估计氧化应激生物标志物与血液 Hg 之间的关系。来自库尼亚雷塞克斯河（Cuniã RESEX）的少年河流水域人群的氧化应激和血液 Hg 水平最高（GST = 27.2（4.93）U/L，MDA = 1.69（0.27）µmol/L，Hg = 20.6（18.0）µg/L）。该人群也具有最高的鱼类食用频率。Hg 与 GST 和 MDA 之间的正相关关系，在调整个体特征后，提示存在氧化作用。本研究表明，氧化应激生物标志物在评估膳食 Hg 暴露中的重要性，因为观察到了初始和可逆的代谢变化，丰富了健康风险评估。
