Center for Wound Healing & Tissue Regeneration, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA.
Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA.
Int J Mol Sci. 2019 Jul 27;20(15):3679. doi: 10.3390/ijms20153679.
Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.
口腔黏膜内的伤口愈合速度比皮肤伤口快。最近的研究表明，微小 RNA 组谱的差异可能是口腔黏膜异常愈合的基础。在这里，我们测试通过增加口腔黏膜特异性 microRNAs 的水平是否可以加速皮肤伤口愈合。基于我们之前发表的皮肤和口腔黏膜伤口愈合配对 miR-seq 数据集，我们确定了一组 57 个差异表达的高表达 microRNA [Sci. Rep. (2019) 9:7160]。这些 microRNAs 根据其表达模式进一步分为 5 个簇，并用 TaqMan 基于对来自伤口边缘 LCM 捕获的上皮细胞的定量来确认其差异表达。在这 5 个簇中，由于其组织特异性表达模式和伤口愈合过程中的时间变化，簇 IV（由 8 个 microRNAs 组成，包括 miR-31）最引人注目。体外功能测定表明，外源性转染 miR-31 可一致增强角质形成细胞的增殖和迁移。在体内，miR-31 模拟物处理可显著加速伤口闭合。我们的结果表明，通过过表达在口腔黏膜特权愈合反应中高度表达的 microRNAs，可以增强皮肤的伤口愈合。
