Dipartimento di Biologia e Biotecnologie "Charles Darwin", Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Roma, Italy.
Department of Biological Science, Southern Methodist University, Dallas, TX 75275, USA.
Int J Mol Sci. 2019 Jul 27;20(15):3691. doi: 10.3390/ijms20153691.
Neural stem progenitor cells (NSPCs) from E13.5 mouse embryos can be maintained in culture under proliferating conditions. Upon growth-factor removal, they may differentiate toward either neuronal or glial phenotypes or both. Exosomes are small extracellular vesicles that are part of the cell secretome; they may contain and deliver both proteins and genetic material and thus play a role in cell-cell communication, guide axonal growth, modulate synaptic activity and regulate peripheral nerve regeneration. In this work, we were interested in determining whether NSPCs and their progeny can produce and secrete extracellular vesicles (EVs) and if their content can affect cell differentiation. Our results indicate that cultured NSPCs produce and secrete EVs both under proliferating conditions and after differentiation. Treatment of proliferating NSPCs with EVs derived from differentiated NSPCs triggers cell differentiation in a dose-dependent manner, as demonstrated by glial- and neuronal-marker expression.
神经干细胞祖细胞（NSPCs）可从 E13.5 期的胚胎中分离，并在增殖条件下于体外培养。在生长因子去除后，NSPCs 可能向神经元或神经胶质细胞表型或两者分化。外泌体是细胞分泌组的一部分，是小的细胞外囊泡；它们可能包含并传递蛋白质和遗传物质，从而在细胞间通讯、引导轴突生长、调节突触活性和调节周围神经再生中发挥作用。在这项工作中，我们感兴趣的是确定 NSPCs 及其祖细胞是否可以产生和分泌细胞外囊泡（EVs），以及它们的内容物是否可以影响细胞分化。我们的结果表明，在增殖条件下和分化后，培养的 NSPCs 都可以产生和分泌 EVs。用来源于分化的 NSPCs 的 EVs 处理增殖的 NSPCs 会以剂量依赖的方式触发细胞分化，这可通过神经胶质和神经元标志物的表达来证明。
