Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China.
Mar Drugs. 2019 Jul 28;17(8):446. doi: 10.3390/md17080446.
Overexpression of the global regulator LaeA in a marine-derived fungal strain of YJ-11 induced obvious morphological changes and metabolic variations. Further chemical investigation of the mutant strain afforded a series of sorbicillinoids including two new ones named 10,11-dihydrobislongiquinolide () and 10,11,16,17-tetrahydrobislongiquinolide (), as well as four known analogues, bislongiquinolide (), 16,17-dihydrobislongiquinolide (), sohirnone A (), and 2',3'-dihydrosorbicillin (). The results support that the global regulator LaeA is a useful tool in activating silent gene clusters in strains to obtain previously undiscovered compounds.
过表达海洋来源真菌 YJ-11 菌株中的全局调控因子 LaeA 会引起明显的形态变化和代谢变化。对突变株的进一步化学研究得到了一系列索比尔菌素类化合物，包括两个新化合物 10,11-二氢比久洛利德（）和 10,11,16,17-四氢比久洛利德（），以及四个已知类似物，比久洛利德（）、16,17-二氢比久洛利德（）、sohirnone A（）和 2',3'-二氢索比尔（）。结果表明，全局调控因子 LaeA 是一种在激活菌株中沉默基因簇以获得以前未发现的化合物的有用工具。
