Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Next to Milad Tower, Tehran, Iran.
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Pathol Res Pract. 2019 Oct;215(10):152556. doi: 10.1016/j.prp.2019.152556. Epub 2019 Jul 23.
Curcumin is a natural non-toxic phenol which is isolated from Curcumin longa L. Mounting evidence has revealed the anticancer properties of curcumin in various tumors, but the underlying molecular mechanisms of this suppression in cervical cancer is still remained unclear. Here we assessed the antitumor effects of curcumin compared with 5-Fluorouracil in Hella cells in spheroids models and monolayer cell cultures. The anti-proliferative effects of curcumin and 5-Fluorouracil were as examined in spheroid and monolayer models. The expression levels of Wnt/β-catenin and NF-kB pathways as well as the influence of the cell cycle were evaluated. Curcumin inhibited cell growth in Hella cells through the regulation of NF-kB and Wnt pathways. Also, cells developed a G2/M cell cycle arrest followed by sub-G1 apoptosis with 5-Fluorouracil and curcumin. It was also shown that curcumin either considerably affects the Wnt/β-catenin and NF-kB pathways. We showed that curcumin inhibits invasion and proliferation of cervical cancer cells via impairment of NF-kB and Wnt/β-catenin pathways, proposing further studies on the potential impacts of this compound on cancer therapy.
姜黄素是一种天然无毒的酚类物质，从姜黄中分离得到。越来越多的证据表明姜黄素在各种肿瘤中有抗癌作用，但宫颈癌中这种抑制作用的潜在分子机制仍不清楚。在这里，我们评估了姜黄素与 5-氟尿嘧啶在 Hella 细胞球体模型和单层细胞培养物中的抗肿瘤作用。在球体和单层模型中检测了姜黄素和 5-氟尿嘧啶的抗增殖作用。评估了 Wnt/β-catenin 和 NF-kB 通路的表达水平以及细胞周期的影响。姜黄素通过调节 NF-kB 和 Wnt 通路抑制 Hella 细胞的生长。此外，用 5-氟尿嘧啶和姜黄素处理后，细胞出现 G2/M 细胞周期阻滞，随后发生亚 G1 凋亡。结果还表明，姜黄素对 Wnt/β-catenin 和 NF-kB 通路有显著影响。我们表明，姜黄素通过抑制 NF-kB 和 Wnt/β-catenin 通路来抑制宫颈癌细胞的侵袭和增殖，这表明需要进一步研究该化合物对癌症治疗的潜在影响。
