Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411004, Maharashtra, India.
Savitribai Phule Pune University, Ganeshkhind, Pune, 411007, Maharashtra, India.
Sci Rep. 2019 Jul 29;9(1):10969. doi: 10.1038/s41598-019-47458-0.
Systemic iron homeostasis dysregulation is primarily associated with inflammation- associated anemia (AI) due to hepcidin up-regulation. Tinospora cordifolia (TC) has shown remarkable anti-inflammatory properties and has been found useful in the treatment of inflammatory disorders. However, the effects and mechanisms of TC on AI have not been studied yet. We conducted in vivo and in vitro studies to evaluate the effect of TC on AI. HPLC studies were also carried out to find out active constituents in TC extract. Model system exhibiting AI was developed by repeated injections of HKBA in Wistar rats. TC treated groups showed significantly higher levels of Hb and RBC count compared to the inflammatory control group. TC treatment showed reduction in the expression of the HAMP (hepcidin) gene in the rat liver. TC extract also inhibited gene expression of inflammatory cytokines (TNF-α, IL-1β) and decreased NO production in RAW 264.7 cells. The HPLC analysis revealed the presence of tinosporaside, which could have synergistically contributed to the above findings. Overall results indicate that TC therapy was able to maintain circulating iron through reduction of inflammatory cytokines and expression of hepcidin in rats.
铁代谢系统性失衡主要与炎症相关贫血（AI）有关，这是由于铁调素的上调。三叶鬼针草（TC）具有显著的抗炎特性，已被证明对治疗炎症性疾病有用。然而，TC 对 AI 的作用和机制尚未得到研究。我们进行了体内和体外研究来评估 TC 对 AI 的影响。还进行了 HPLC 研究以找出 TC 提取物中的活性成分。通过在 Wistar 大鼠中反复注射 HKBA 来建立表现出 AI 的模型系统。与炎症对照组相比，TC 治疗组的 Hb 和 RBC 计数明显更高。TC 治疗显示大鼠肝脏中 HAMP（铁调素）基因的表达减少。TC 提取物还抑制了 RAW 264.7 细胞中炎症细胞因子（TNF-α，IL-1β）的基因表达并减少了 NO 的产生。HPLC 分析表明存在替诺帕萨德，这可能对上述发现有协同作用。总体结果表明，TC 治疗能够通过减少大鼠体内炎症细胞因子的表达和铁调素的表达来维持循环铁。
