Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Crops, Hainan University, Haikou, 570228, China.
Hainan Academy of Agricultural Sciences, Haikou, 571100, China.
Sci Rep. 2019 Jul 29;9(1):10991. doi: 10.1038/s41598-019-47518-5.
Guar gum is an important raw material in the food, textile and oil industries, but the biosynthesis of guar gum remains unclear. To illuminate the genes involved in guar gum biosynthesis, guar beans from 30 and 40 days after flowering (DAF) were used for RNA sequencing in this study. A total of 2,535 and 2,724 preferentially expressed genes were found in 30 and 40 DAF endosperm, and 3,720 and 2,530 preferentially expressed genes were found in 30 and 40 DAF embryos, respectively. Of these, mannan synthase genes, α-galactosyltransferase genes and cellulose synthase genes were preferentially expressed in the endosperm from 30 and 40 DAF. The high expression level of these glycometabolism genes in endosperm is consistent with the expectation that the main component of guar gum is galactomannan. We believe that genes related to guar gum biosynthesis found in this study will be useful for both new variety development via genetic engineering and synthetic biology research on guar gum biosynthesis in the future.
瓜尔胶是食品、纺织和石油工业的重要原料，但瓜尔胶的生物合成仍不清楚。为了阐明参与瓜尔胶生物合成的基因，本研究使用开花后 30 天和 40 天（DAF）的瓜尔豆进行 RNA 测序。在 30 和 40 DAF 胚乳中分别发现了 2535 和 2724 个优先表达基因，在 30 和 40 DAF 胚胎中分别发现了 3720 和 2530 个优先表达基因。其中，甘露聚糖合酶基因、α-半乳糖基转移酶基因和纤维素合酶基因在 30 和 40 DAF 的胚乳中优先表达。这些糖代谢基因在胚乳中的高表达水平与瓜尔胶的主要成分是半乳甘露聚糖的预期一致。我们相信，本研究中发现的与瓜尔胶生物合成相关的基因，将对未来通过基因工程进行新品种开发和瓜尔胶生物合成的合成生物学研究都具有重要意义。
