Department of Physical and Analytical Chemistry, University of Oviedo, c/ Julián Clavería 8, 33006, Oviedo, Spain.
Department of Physics, University of Oviedo, 33204, Gijón, Spain.
Anal Bioanal Chem. 2019 Oct;411(25):6615-6624. doi: 10.1007/s00216-019-02031-6. Epub 2019 Jul 30.
Histamine, a biogenic amine, is abundant in fermented foods and beverages, notably wine. A high intake of this monoamine may produce adverse reactions in humans, which may be severe in individuals with a reduced capacity to catabolise extrinsic histamine. Thus, control of histamine concentration during wine production and before distribution is advisable. Simple, rapid, point-of-use bioanalytical platforms are needed because traditional methods for the detection and quantification of histamine are expensive and time-consuming. This work applies the lateral flow immunoassay technique to histamine detection. Superparamagnetic particle labels, and an inductive sensor designed to read the test line in the immunoassay, enable magnetic quantification of the molecule. The system is calibrated with histamine standards in the interval of interest for wine production. A commercial optical strip reader is used for comparison measurements. The lateral flow system has a limit of detection of 1.2 and 1.5 mg/L for the inductive and optical readers, respectively. The capability of the inductive system for histamine quantification is demonstrated for wine samples at different processing points (at the end of alcoholic fermentation, at the end of malolactic fermentation, in freshly bottled wine, and in reserve wine). The results are validated by ultra-high-performance liquid chromatography. Graphical abstract.
组胺是一种生物胺，在发酵食品和饮料中含量丰富，尤其是葡萄酒。这种单胺的大量摄入可能会在人类中产生不良反应，而对于外源性组胺代谢能力降低的个体，这些反应可能会很严重。因此，在葡萄酒生产和分销之前，控制组胺浓度是明智的。由于传统的组胺检测和定量方法既昂贵又耗时，因此需要简单、快速、现场使用的生物分析平台。本工作应用侧向流动免疫分析技术检测组胺。超顺磁颗粒标记物和设计用于读取免疫分析测试线的感应传感器，使分子的磁性定量成为可能。该系统使用生产葡萄酒感兴趣的范围内的组胺标准品进行校准。商业光学条带阅读器用于比较测量。对于感应和光学读数器，侧向流动系统的检测限分别为 1.2 和 1.5mg/L。感应系统用于定量葡萄酒样品中不同处理点（酒精发酵结束时、苹果酸-乳酸发酵结束时、新鲜装瓶的葡萄酒和陈酿葡萄酒）中的组胺的能力得到了验证。结果通过超高效液相色谱法进行验证。
